假交替单胞菌 2515 的全基因组测序揭示了其对鳗弧菌的抗菌效力:一项初步的无菌研究。

IF 1.8 3区 生物学 Q4 MICROBIOLOGY
Fenglin Wang, Abdallah Ghonimy, Xiuhua Wang
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引用次数: 0

摘要

从万年青养殖水中分离出的假交替单胞菌(Pseudoalteromonas piscicida 2515)是一种潜在的海洋益生菌,具有广泛的抗弧菌特性。然而,有关 P. piscicida 2515 的基因组信息很少。本研究分析了 P. piscicida 2515 菌株的一般基因组特征和益生特性。此外,我们还通过扫描电子显微镜(SEM)确定了该菌株的抗菌机制。结果表明,P. piscicida 2515 的全基因组序列包含一个染色体和一个质粒,总长度为 5,541,406 bp,G + C 含量为 43.24%,预测了 4679 个编码蛋白质的基因。利用基因组挖掘工具发现了各种粘附相关基因、氨基酸和维生素代谢及生物合成基因以及应激反应基因。几丁质、溴环肽、兰特生物素和半乳肽编码基因的存在表明,P. piscicida 2515 菌株具有很强的抗菌活性。此外,在与鳗弧菌(Vibrio anguillarum)的共培养过程中,P. piscicida 2515 的表面显示出囊泡/脓疱状结构,这可能参与了其杀菌活性,代表了一种抗菌机制。此外,还注释了 16 个溶血基因和 3 个抗生素耐药基因,包括四环素、氟喹诺酮和碳青霉烯,但未检测到编码肠毒素 FM(entFM)、cereulide(ces)和细胞毒素 K 的毒力基因。为确认 P. piscicida 2515 的安全特性,应进行进一步的测试,包括长期毒理学测试、生态毒理学评估和抗生素耐药性转移风险评估。我们的研究结果揭示了对 P. piscicida 2515 的益生菌特性和抗菌机制的新认识,并为其在水产养殖中的应用提供了理论信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Whole-genome sequencing of Pseudoalteromonas piscicida 2515 revealed its antibacterial potency against Vibrio anguillarum: a preliminary invitro study

Whole-genome sequencing of Pseudoalteromonas piscicida 2515 revealed its antibacterial potency against Vibrio anguillarum: a preliminary invitro study

Pseudoalteromonas piscicida 2515, isolated from Litopenaeus vannamei culture water, is a potential marine probiotic with broad anti-Vibrio properties. However, genomic information on P. piscicida 2515 is scarce. In this study, the general genomic characteristics and probiotic properties of the P. piscicida 2515 strain were analysed. In addition, we determined the antibacterial mechanism of this bacterial strain by scanning electron microscopy (SEM). The results indicated that the whole-genome sequence of P. piscicida 2515 contained one chromosome and one plasmid, including a total length of 5,541,406 bp with a G + C content of 43.24%, and 4679 protein-coding genes were predicted. Various adhesion-related genes, amino acid and vitamin metabolism and biosynthesis genes, and stress-responsive genes were found with genome mining tools. The presence of genes encoding chitin, bromocyclic peptides, lantibiotics, and sactipeptides showed the strong antibacterial activity of the P. piscicida 2515 strain. Moreover, in coculture with Vibrio anguillarum, P. piscicida 2515 displayed vesicle/pilus-like structures located on its surface that possibly participated in its bactericidal activity, representing an antibacterial mechanism. Additionally, 16 haemolytic genes and 3 antibiotic resistance genes, including tetracycline, fluoroquinolone, and carbapenem were annotated, but virulence genes encoding enterotoxin FM (entFM), cereulide (ces), and cytotoxin K were not detected. Further tests should be conducted to confirm the safety characteristics of P. piscicida 2515, including long-term toxicology tests, ecotoxicological assessment, and antibiotic resistance transfer risk assessment. Our results here revealed a new understanding of the probiotic properties and antibacterial mechanism of P. piscicida 2515, in addition to theoretical information for its application in aquaculture.

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来源期刊
CiteScore
5.60
自引率
11.50%
发文量
104
审稿时长
3 months
期刊介绍: Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.
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