从黑土中分离出来的 Methylobacterium nigriterrae sp.

IF 1.8 3区 生物学 Q4 MICROBIOLOGY
Le-Bin Chen, Yu-Ting OuYang, Lan Liu, Pin-Jiao Jin, Rong-Rong Huang, Wen-Yi Pan, Ying Wang, Jia-Ying Xing, Ting-Ting She, Jian-Yu Jiao, Shuang Wang, Wen-Jun Li
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引用次数: 0

摘要

从中国黑龙江省哈尔滨市的黑土样本中分离出了一种需氧、革兰氏染色阴性、运动棒状杆菌,命名为 SYSU BS000021T。基于 16S rRNA 基因序列的系统进化分析表明,该分离菌属于甲基杆菌属,与 Methylobacterium segetis KCTC 62267 T(98.51%)和 Methylobacterium oxalidis DSM 24028 T(97.79%)的序列相似度最高。生长温度为 20-37℃(最适温度为 28℃),pH 值为 6.0-8.0(最适 pH 值为 7.0),存在 0%(w/v)NaCl。极性脂质包括磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油、一种不明氨基脂质和一种不明极性脂质。主要的细胞脂肪酸(> 5%)为 C18:0 和 C18:1 ω7c 和/或 C18:1 ω6c。主要的呼吸醌是 Q-10。根据全基因组分析,基因组 G + C 含量为 68.36%。SYSU BS000021T 菌株与 Methylobacterium 属其他成员之间的平均核苷酸同一性(≤ 83.5%)和数字 DNA-DNA 杂交(≤ 27.3%)值均低于区分新型原核生物物种的推荐阈值。根据表型学、化学分类学和系统发生学分析的结果,SYSU BS000021T 菌株代表了 Methylobacterium 属的一个新菌种,拟命名为 Methylobacterium nigriterrae sp.该新物种的模式菌株为 SYSU BS000021T(= GDMCC 1.3814 T = KCTC 8051 T)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Methylobacterium nigriterrae sp. nov., isolated from black soil

Methylobacterium nigriterrae sp. nov., isolated from black soil

Methylobacterium nigriterrae sp. nov., isolated from black soil

An aerobic, Gram-stain-negative, motile rod bacterium, designated as SYSU BS000021T, was isolated from a black soil sample in Harbin, Heilongjiang province, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the genus Methylobacterium, and showed the highest sequence similarity to Methylobacterium segetis KCTC 62267 T (98.51%) and Methylobacterium oxalidis DSM 24028 T (97.79%). Growth occurred at 20–37℃ (optimum, 28 °C), pH 6.0–8.0 (optimum, pH 7.0) and in the presence of 0% (w/v) NaCl. Polar lipids comprised of phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified aminolipid and one unidentified polar lipid. The major cellular fatty acids (> 5%) were C18:0 and C18:1 ω7c and/or C18:1 ω6c. The predominant respiratory quinone was Q-10. The genomic G + C content was 68.36% based on the whole genome analysis. The average nucleotide identity (≤ 83.5%) and digital DNA–DNA hybridization (≤ 27.3%) values between strain SYSU BS000021T and other members of the genus Methylobacterium were all lower than the threshold values recommended for distinguishing novel prokaryotic species. Based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain SYSU BS000021T represents a novel species of the genus Methylobacterium, for which the name Methylobacterium nigriterrae sp. nov. is proposed. The type strain of the proposed novel species is SYSU BS000021T (= GDMCC 1.3814 T = KCTC 8051 T).

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来源期刊
CiteScore
5.60
自引率
11.50%
发文量
104
审稿时长
3 months
期刊介绍: Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.
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