长非编码 RNA FLG-AS1 通过负向调节 miR-147b 抑制宫颈癌进展

IF 1.1 4区医学 Q4 MEDICAL LABORATORY TECHNOLOGY

Annals of clinical and laboratory science Pub Date : 2024-03-01

Mengying Gao, Xixia Pang, Suna Ni, Zhixia Wei, Dandan Li

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引用次数: 0

摘要

研究目的该研究探讨了FLG-AS1与宫颈癌预后的关系以及FLG-AS1与miR-147b之间的相互作用机制，以确定宫颈癌的潜在治疗靶点：方法：本研究获得了125例宫颈癌患者的组织样本和临床病理特征。聚合酶链反应法检测样本中 FLG-AS1 的表达水平。CCK-8和Transwell试验用于评估FLG-AS1对宫颈癌细胞增殖和转移的影响。FLG-AS1和miR-147b的作用机制通过双荧光素酶报告基因实验进行了探究。通过一系列统计方法探讨了FLG-AS1在宫颈癌中的预后作用：结果：在宫颈癌细胞和组织中，FLG-AS1的表达明显下调。结果：在宫颈癌细胞和组织中，FLG-AS1 的表达明显下调，FLG-AS1 通过负调控 miR-147b 的表达来抑制宫颈癌细胞的活性。结论：FLG-AS1表达较低时，宫颈癌患者的预后较差：结论：FLG-AS1可被视为一种新型的宫颈癌预后生物标志候选物，它通过负向调节miR-147b来影响癌细胞的进展。

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Long Non-Coding RNA FLG-AS1 Inhibits Cervical Cancer Progression through Negatively Modulating miR-147b.

Objective: The study investigated the association between FLG-AS1 and cervical cancer prognosis and the interaction mechanism between FLG-AS1 and miR-147b in order to identify potential therapeutic targets for cervical cancer.

Methods: In this study, tissue samples and clinicopathological characteristics were obtained from 125 cervical cancer patients. FLG-AS1 expression levels in the samples were detected by polymerase chain reaction assay. CCK-8 and Transwell assays were used to evaluate FLG-AS1's impact on cervical cancer cell proliferation and metastasis. The mechanism of action of FLG-AS1 and miR-147b was probed by a dual luciferase reporter gene assay. The prognostic nature of FLG-AS1 in cervical cancer was explored by a series of statistical approaches.

Results: In cervical cancer cells and tissues, FLG-AS1 expression is markedly downregulated. FLG-AS1 inhibits the activities of cervical cancer cells by negatively regulating miR-147b expression. Patients with cervical cancer have a poor prognosis when FLG-AS1 expression is low.

Conclusion: FLG-AS1 may be considered as a novel cervical cancer prognostic biomarker candidate, which affects cancer cell progression by negatively regulating miR-147b.

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来源期刊

Annals of clinical and laboratory science 医学-医学实验技术

CiteScore

1.60

自引率

0.00%

发文量

112

审稿时长

6-12 weeks

期刊介绍： The Annals of Clinical & Laboratory Science welcomes manuscripts that report research in clinical science, including pathology, clinical chemistry, biotechnology, molecular biology, cytogenetics, microbiology, immunology, hematology, transfusion medicine, organ and tissue transplantation, therapeutics, toxicology, and clinical informatics.