亚速尔群岛分离的六株细菌异型线虫的生物控制潜力。

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
R Beltrí, H R Monteiro, D Toubarro, N Simões, A Garriga
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引用次数: 0

摘要

昆虫病原线虫(EPNs)与粳稻密切相关,有可能被用作粳稻的生物防治剂,但实地结果并不一致,因此需要不断寻找更适应环境的本地 EPNs。因此,我们在亚速尔群岛进行了调查,以分离出新的细菌异habditis 菌株,并在实验室条件下评估它们对模式生物Galleria mellonella的毒力。从牧场和沿海环境中获得了六株菌株,并对线虫和共生细菌进行了分子鉴定。生物测定结果表明,Az172、Az186 和 Az171 在致死剂量(LD50)测定和短时间接触实验中表现出较高的毒力,与 Az29 的毒力相当。72 小时后,这些毒株的平均致死剂量为 11 感染幼虫 cm-2,致死时间(LT50)为 34 小时,初始暴露时间仅为 60 分钟,死亡率为 40%。Az170表现中等,而Az179和Az180则被归类为低毒性菌株。不过,这两种菌株的繁殖潜力最高,平均每克幼虫可感染 1700 只幼虫。对所获得的本地 EPNs 进行的生物测定表明,这些菌株具有高毒力和适应当地环境条件的特点,因此有潜力用于针对粳稻的生物防治措施。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Biocontrol potential of six Heterorhabditis bacteriophora strains isolated in the Azores Archipelago.

Entomopathogenic nematodes (EPNs) are closely associated with Popillia japonica and potentially used as their biological control agents, although field results proved inconsistent and evoked a continual pursuit of native EPNs more adapted to the environment. Therefore, we surveyed the Azorean Archipelago to isolate new strains of Heterorhabditis bacteriophora and to evaluate their virulence against the model organism Galleria mellonella under laboratory conditions. Six strains were obtained from pasture and coastal environments and both nematode and symbiont bacteria were molecularly identified. The bioassays revealed that Az172, Az186, and Az171 presented high virulence across the determination of a lethal dose (LD50) and short exposure time experiments with a comparable performance to Az29. After 72 hours, these virulent strains presented a mean determination of a lethal dose of 11 infective juveniles cm-2, a lethal time (LT50) of 34 hours, and achieved 40% mortality after an initial exposure time of only 60 minutes. Az170 exhibited an intermediate performance, whereas Az179 and Az180 were classified as low virulent strains. However, both strains presented the highest reproductive potential with means of 1700 infective juveniles/mg of larvae. The bioassays of the native EPNs obtained revealed that these strains hold the potential to be used in biological control initiatives targeting P. japonica because of their high virulence and locally adapted to environmental conditions.

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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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