将氧化应激和NF-KB/iNOS炎症通路作为诊断溺水的创新生物标志物,并将其与大鼠在淡水和盐水中的死后浸泡区分开来。

IF 2.2 3区 医学 Q1 MEDICINE, LEGAL
International Journal of Legal Medicine Pub Date : 2024-09-01 Epub Date: 2024-05-27 DOI:10.1007/s00414-024-03249-5
Rana Adel, Manar Fouli Gaber Ibrahim, Samar Hisham Elsayed, Nada A Yousri
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引用次数: 0

摘要

背景:由于溺水的病理生理学十分复杂,因此在水中发现尸体会引发一个有关将死因确定为溺水的问题。此外,尸体可能在死后被淹没:评估氧化应激标志物和 NF-KB/iNOS 炎症通路作为溺水诊断生物标志物的作用,以及它们是否能区分淡水溺水和海水溺水:本研究将45只成年雄性白化大鼠分为5组:对照组(C)、淡水溺水组(FD)、淡水死后浸泡组(FPS)、盐水溺水组(SD)和盐水死后浸泡组(SPS)。解剖后,对各组大鼠的肺部进行组织学、免疫组化(Caspase 3、TNF-α、NF-kB、COX-2 和 iNOS)和生化研究;MDA、NOx、SOD、GSH、VCAM-1、COX-2;以及 RT-PCR 对 NF-kB 和 iNOS 基因表达进行相对定量:结果:溺水组的肺氧化标记物明显受影响,高于死后浸泡组。溺水组的炎症通路标记物也明显增加,值得关注的是,盐水溺水组的所有标记物受到的影响都明显大于淡水溺水组:结论:测试的标记物可用于准确诊断溺水,并将其与死后浸水区分开来,同时更好地了解溺水死亡的机制,因为炎症和氧化应激这两种机制都被揭示并参与其中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Oxidative stress and NF-KB/iNOS inflammatory pathway as innovative biomarkers for diagnosis of drowning and differentiating it from postmortem submersion in both fresh and saltwater in rats.

Oxidative stress and NF-KB/iNOS inflammatory pathway as innovative biomarkers for diagnosis of drowning and differentiating it from postmortem submersion in both fresh and saltwater in rats.

Background: Finding a dead body in water raises an issue concerning determining the cause of death as drowning because of the complex pathophysiology of drowning. In addition, the corpse may be submersed postmortem.

Objective: Evaluate the role of oxidative stress markers and NF-KB/iNOS inflammatory pathway as diagnostic biomarkers in drowning and whether they could differentiate freshwater from saltwater drowning.

Methods: This study included forty-five adult male albino rats classified into five groups: control group (C), Freshwater-drowned group (FD), Freshwater postmortem submersion group (FPS), saltwater-drowned group (SD), and saltwater postmortem submersion group (SPS). After the autopsy, the rats' lungs in each group were prepared for histological, immunohistochemical (caspase 3, TNF-α, NF-kB, COX-2 & iNOS), biochemical studies; MDA, NOx, SOD, GSH, VCAM-1, COX-2; and RT-PCR for the relative quantification of NF-kB and iNOS genes expression.

Results: Lung oxidative markers were significantly affected in drowned groups than in postmortem submersion groups. Inflammatory pathway markers were also significantly increased in the drowned groups, with concern that all markers were significantly affected more in saltwater than in freshwater drowned group.

Conclusions: It is concluded that the tested markers can be used accurately in diagnosing drowning and differentiating it from postmortem submersion with a better understanding of the mechanism of death in drowning as both mechanisms, inflammatory and oxidative stress, were revealed and involved.

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来源期刊
CiteScore
5.80
自引率
9.50%
发文量
165
审稿时长
1 months
期刊介绍: The International Journal of Legal Medicine aims to improve the scientific resources used in the elucidation of crime and related forensic applications at a high level of evidential proof. The journal offers review articles tracing development in specific areas, with up-to-date analysis; original articles discussing significant recent research results; case reports describing interesting and exceptional examples; population data; letters to the editors; and technical notes, which appear in a section originally created for rapid publication of data in the dynamic field of DNA analysis.
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