大肠杆菌和毕赤菌:生产全长 IgG 的微生物细胞工厂平台。

IF 8.1 2区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Critical Reviews in Biotechnology Pub Date : 2025-02-01 Epub Date: 2024-05-26 DOI:10.1080/07388551.2024.2342969
Shyam Krishna, Sang Taek Jung, Eun Yeol Lee
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引用次数: 0

摘要

由于需求得不到满足,制药业正在研究哺乳动物细胞以外的宿主,以生产用于各种治疗和研究的抗体。尽管存在一些缺点,大肠杆菌和毕赤菌仍是生产抗体的首选微生物宿主。尽管在大肠杆菌中已成功生产出全长抗体,但大肠杆菌大多用于生产抗体片段,如抗原结合片段(Fab)、单链片段变量(scFv)和纳米抗体。相比之下,真核微生物宿主 Pichia 大多用于生产糖基化的全长抗体,但高甘露糖基化是一大挑战。先进的策略,如在内毒素编辑的大肠杆菌中引入类人糖基化和基于无细胞系统的糖基化,正在这些微生物中创建类人糖基化抗体谱方面取得进展。这篇综述首先解释了抗体的结构和功能要求,然后描述和分析了大肠杆菌和棒状杆菌作为宿主提供有利环境以创造全功能抗体的潜力。此外,作者还比较了这些微生物的某些特征,并预测了它们在抗体生产中的前景。简而言之,这篇综述分析、比较并强调了大肠杆菌和牧杆菌作为生产抗体的潜在宿主。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Escherichia coli and Pichia pastoris: microbial cell-factory platform for -full-length IgG production.

Owing to the unmet demand, the pharmaceutical industry is investigating an alternative host to mammalian cells to produce antibodies for a variety of therapeutic and research applications. Regardless of some disadvantages, Escherichia coli and Pichia pastoris are the preferred microbial hosts for antibody production. Despite the fact that the production of full-length antibodies has been successfully demonstrated in E. coli, which has mostly been used to produce antibody fragments, such as: antigen-binding fragments (Fab), single-chain fragment variable (scFv), and nanobodies. In contrast, Pichia, a eukaryotic microbial host, is mostly used to produce glycosylated full-length antibodies, though hypermannosylated glycan is a major challenge. Advanced strategies, such as the introduction of human-like glycosylation in endotoxin-edited E. coli and cell-free system-based glycosylation, are making progress in creating human-like glycosylation profiles of antibodies in these microbes. This review begins by explaining the structural and functional requirements of antibodies and continues by describing and analyzing the potential of E. coli and P. pastoris as hosts for providing a favorable environment to create a fully functional antibody. In addition, authors compare these microbes on certain features and predict their future in antibody production. Briefly, this review analyzes, compares, and highlights E. coli and P. pastoris as potential hosts for antibody production.

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来源期刊
Critical Reviews in Biotechnology
Critical Reviews in Biotechnology 工程技术-生物工程与应用微生物
CiteScore
20.80
自引率
1.10%
发文量
71
审稿时长
4.8 months
期刊介绍: Biotechnological techniques, from fermentation to genetic manipulation, have become increasingly relevant to the food and beverage, fuel production, chemical and pharmaceutical, and waste management industries. Consequently, academic as well as industrial institutions need to keep abreast of the concepts, data, and methodologies evolved by continuing research. This journal provides a forum of critical evaluation of recent and current publications and, periodically, for state-of-the-art reports from various geographic areas around the world. Contributing authors are recognized experts in their fields, and each article is reviewed by an objective expert to ensure accuracy and objectivity of the presentation.
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