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引用次数: 0
摘要
核糖体蛋白 L13（RPL13）在进化过程中高度保守。目前，RPL13在昆虫中的特性和功能尚未得到表征。在这项研究中，首次发现森蝽 RPL13（BmRPL13）被特异性地招募到紫外线（UV）诱导的 DNA 损伤位点，并有助于紫外线损伤的修复。表达 BmRPL13 的大肠杆菌对紫外线辐射有更好的抵抗力。在 BmN 细胞中敲除 BmRPL13 的表达后，紫外线损伤 DNA 的修复速度减慢。进一步的研究结果表明，BmRPL13与B. mori nucleopolyhedrovirus（BmNPV）ORF65（Bm65）蛋白相互作用，定位于BmNPV的紫外线诱导DNA损伤位点，帮助修复紫外线损伤的病毒DNA。
Bombyx mori RPL13 participates in UV-induced DNA damage repair of B. mori nucleopolyhedrovirus through interaction with Bm65
Ribosomal protein L13 (RPL13) is highly conserved in evolution. At present, the properties and functions of RPL13 have not been characterised in insects. In this study, Bombyx mori RPL13 (BmRPL13) was first found to be specifically recruited to the sites of ultraviolet (UV)-induced DNA damage and contributed to UV damage repair. Escherichia coli expressing BmRPL13 showed better resistance to UV radiation. After knocking down the expression of BmRPL13 in BmN cells, the repair speed of UV-damaged DNA slowed down. The further results showed that BmRPL13 interacted with B. mori nucleopolyhedrovirus (BmNPV) ORF65 (Bm65) protein to locate at the UV-induced DNA damage sites of BmNPV and helped repair UV-damaged viral DNA.
期刊介绍：
Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins.
This includes research related to:
• insect gene structure
• control of gene expression
• localisation and function/activity of proteins
• interactions of proteins and ligands/substrates
• effect of mutations on gene/protein function
• evolution of insect genes/genomes, especially where principles relevant to insects in general are established
• molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations
• gene mapping using molecular tools
• molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects
Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).
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