废水中普遍存在耐多药弯曲杆菌:环境和公共卫生问题的威胁。

IF 4.3 2区 医学 Q1 GASTROENTEROLOGY & HEPATOLOGY
Helicobacter Pub Date : 2024-05-26 DOI:10.1111/hel.13095
Olufunmilayo Modupe Oluwakoya, Anthony Ifeanyi Okoh
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引用次数: 0

摘要

污水中普遍存在的耐多药弯曲杆菌给环境可持续性和公共卫生带来了严峻的挑战。本研究考察了南非东开普省废水中存在的耐多药弯曲杆菌及其对环境生态系统和公共卫生的影响。2022 年 4 月至 9 月期间,在该省不同地点收集了 45 份家庭污水(HHE)和污水处理厂污水(WWTPE)样本。在 HHE 和 WWTPE 中,推定弯曲杆菌属的计数范围分别为 5.2 × 103 至 6.03 × 104 CFU/mL,4.93 × 103 至 1.04 × 104 CFU/mL。约 42.55% 的样本对弯曲杆菌呈阳性。所有分离物中都检测到了五种毒力决定因子,包括 cadF 和 wlaN;然而,flgR(19.23%)、ciaB 和 ceuE(15.38%)的流行率较低。确诊弯曲杆菌分离物的抗生素图谱显示,对所有测试抗生素的耐药性都很高(>55%),从 55.77%(萘啶酸)到 92.30%(红霉素)不等,对其他抗生素的耐药性依次为环丙沙星(51.92%)、阿奇霉素(50%)和左氧氟沙星(48.08%)。相反,庆大霉素对 61.54% 的分离株敏感,其次是亚胺培南(57.69%)和链霉素(51.92%)。WWTPE 的抗生素耐药性指数(ARI)为 0.19,低于克鲁姆伯曼允许的 0.2 临界值;而 HHE 的抗生素耐药性指数较高。分离物各自的多重抗生素耐药性指数(MARI)介于 0.08 和 1.00 之间。在检测的表型耐药弯曲杆菌分离物中,发现了 21 个耐药基因,分别编码对 β-内酰胺类、碳青霉烯类、氨基糖苷类、酚类、喹诺酮类、四环素类和大环内酯类的耐药性,这也解释了本研究中观察到的表型耐药性。本研究的结论是,研究地区的废水是具有多种耐药性和潜在致病性弯曲杆菌的重要贮存地,这表明有必要对废水进行适当处理,以消除废水中的生物,然后再将最终废水排放到受纳流域。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Prevalence of multidrug-resistant Campylobacter species in wastewater effluents: A menace of environmental and public health concern

Prevalence of multidrug-resistant Campylobacter species in wastewater effluents: A menace of environmental and public health concern

The prevalence of multidrug-resistant Campylobacter species in wastewater effluents presents a formidable challenge at the intersection of environmental sustainability and public health. This study examined the presence of multidrug-resistant Campylobacter in wastewater effluents in the Eastern Cape Province, South Africa, and its implications for environmental ecosystems and public health. Forty-five samples from household effluent (HHE) and wastewater treatment plant effluent (WWTPE) were collected at different geographical locations within the province between April and September 2022. The counts of the presumptive Campylobacter genus ranged from 5.2 × 103 to 6.03 × 104 CFU/mL for HHE and 4.93 × 103 to 1.04 × 104 CFU/mL for WWTPE. About 42.55% of the samples were positive for Campylobacter species. Five virulence determinants including the cadF and wlaN were detected in all the isolates; however, flgR (19.23%), ciaB, and ceuE (15.38%) were less prevalent. The antibiogram profiles of confirmed Campylobacter isolates revealed high resistance (>55%) against all tested antibiotics ranging from 55.77% (nalidixic acid) to 92.30% (erythromycin), and resistance against the other antibiotics followed the order ciprofloxacin (51.92%), azithromycin (50%), and levofloxacin (48.08%). On the contrary, gentamicin was sensitive against 61.54% of the isolates, followed by imipenem (57.69%) and streptomycin (51.92%). The WWTPE's antibiotic resistance index (ARI) was 0.19, lower than the permitted Krumperman threshold of 0.2; and HHE's ARIs were higher. The isolates' respective multiple antibiotic resistance indexes (MARI) varied between 0.08 and 1.00. Among the phenotypically resistant Campylobacter isolates examined, 21 resistance determinants encoding resistance against β-lactam, carbapenems, aminoglycosides, phenicol, quinolones, tetracyclines, and macrolides were detected, which explains the phenotypic resistance observed in the study. This study concludes that the wastewaters in the study areas are important reservoirs of multidrug-resistant and potentially pathogenic Campylobacter species, suggesting the need for proper treatment of the wastewaters to eliminate the organisms in the effluents before discharge the final effluent to the receiving watershed.

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来源期刊
Helicobacter
Helicobacter 医学-微生物学
CiteScore
8.40
自引率
9.10%
发文量
76
审稿时长
2 months
期刊介绍: Helicobacter is edited by Professor David Y Graham. The editorial and peer review process is an independent process. Whenever there is a conflict of interest, the editor and editorial board will declare their interests and affiliations. Helicobacter recognises the critical role that has been established for Helicobacter pylori in peptic ulcer, gastric adenocarcinoma, and primary gastric lymphoma. As new helicobacter species are now regularly being discovered, Helicobacter covers the entire range of helicobacter research, increasing communication among the fields of gastroenterology; microbiology; vaccine development; laboratory animal science.
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