高温和氢化可的松对成纤维细胞增殖的影响

IF 2.1 4区 生物学 Q4 CELL BIOLOGY
Histochemistry and Cell Biology Pub Date : 2024-09-01 Epub Date: 2024-05-27 DOI:10.1007/s00418-024-02295-9
Zuzana Pavlikova, Oldrich Zahradnicek, Anna Jelinek Michaelidesova, Jaromir Sramek, Marie Davidkova, Maria Hovorakova
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引用次数: 0

摘要

高热和氢化可的松(HC)已被证实是致畸剂,会对怀孕早期的胚胎发育产生负面影响。细胞增殖是早期胚胎发育的主要过程之一。本研究的重点是测试温度升高和添加 HC 对体外培养细胞增殖的影响。用 HC 处理 V79-4 细胞系,并分别在 37 ℃ 或 39 ℃ 下进行体外培养。为了揭示这两种因素的影响,我们采用多种方法(菌落形成检测、生成生长曲线、计算倍增时间和有丝分裂指数估算)评估了在不同条件下培养的细胞的增殖情况。我们的结果表明,短期暴露于高温会轻微刺激细胞增殖,而长期暴露于高温则会抑制细胞增殖。然而,碳氢化合物(0.1 毫克/毫升)会刺激细胞增殖。有趣的是,HC 和长期暴露于高温(39 °C)的相互作用至少部分补偿了高温的负面影响,与在 39 °C下培养而不添加 HC 的细胞相比,增殖率更高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Effect of elevated temperature and hydrocortisone addition on the proliferation of fibroblasts.

Effect of elevated temperature and hydrocortisone addition on the proliferation of fibroblasts.

Hyperthermia along with hydrocortisone (HC) are proven teratogens that can negatively influence embryo development during early pregnancy. Proliferation of cells is one of the main developmental processes during the early embryogenesis. This study was focused on testing the effect of elevated temperature and HC addition on proliferation of cells in in vitro cultures. The V79-4 cell line was treated with HC and cultured in vitro at 37 °C or 39 °C, respectively. To reveal the effect of both factors, the proliferation of cells cultured under different conditions was evaluated using various approaches (colony formation assay, generation of growth curves, computation of doubling times, and mitotic index estimation). Our results indicate that a short-term exposure to elevated temperature slightly stimulates and a long-term exposure suppresses cell proliferation. However, HC (0.1 mg/ml) acts as a stimulator of cell proliferation. Interestingly, the interaction of HC and long-term elevated temperature (39 °C) exposure results in at least partial compensation of the negative impact of elevated temperature by HC addition and in higher proliferation if compared with cells cultured at 39 °C without addition of HC.

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来源期刊
Histochemistry and Cell Biology
Histochemistry and Cell Biology 生物-细胞生物学
CiteScore
4.90
自引率
8.70%
发文量
112
审稿时长
1 months
期刊介绍: Histochemistry and Cell Biology is devoted to the field of molecular histology and cell biology, publishing original articles dealing with the localization and identification of molecular components, metabolic activities and cell biological aspects of cells and tissues. Coverage extends to the development, application, and/or evaluation of methods and probes that can be used in the entire area of histochemistry and cell biology.
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