A A Agnihotri, S P Awandkar, R C Kulkarni, S G Chavhan, R D Suryawanshi, A Mohan, M B Kulkarni, V G Chavan
{"title":"印度西部和中部地区流行的传染性法氏囊病病毒的分离和分子特征。","authors":"A A Agnihotri, S P Awandkar, R C Kulkarni, S G Chavhan, R D Suryawanshi, A Mohan, M B Kulkarni, V G Chavan","doi":"10.22099/IJVR.2023.47037.6774","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Infectious bursal disease (IBD) caused substantial economic loss in central and western India during 2020 and 2021.</p><p><strong>Aims: </strong>The study was conducted to characterize IBD virus (IBDV) from field outbreaks.</p><p><strong>Methods: </strong>The study was conducted on 360 samples from 42 poultry flocks. The samples were subjected to histopathology and molecular detection, followed by phylogenetic typing of the partial <i>VP2</i> gene.</p><p><strong>Results: </strong>The mortality ranged from 15.25 to 60.18%. The necropsy showed hemorrhages on thigh muscles, mottled spleen, swelling of kidneys and bursae with hemorrhages, and cheesy exudate. Histopathology revealed extensive necrosis and depletion of follicular lymphoid cells within the cortex and medulla, along with widespread hemorrhages, edema, and cystic cavities in the bursa. The field isolates showed cytopathic effects in the seventh passage. The cytopathic effects included swelling, rounding, granulation of cytoplasm around the nucleus, fragmentation of the infected cells, and detachment. The reverse transcription-polymerase chain reaction amplified 664 bp partial <i>VP2</i> gene. The phylogenetic analysis identified 19 field isolates as very virulent IBDVs (vvIBDVs) and three as classical strains.</p><p><strong>Conclusion: </strong>The results indicated that different vvIBDV strains are involved in disease outbreaks in central and western India.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"24 4","pages":"345-350"},"PeriodicalIF":0.8000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11127732/pdf/","citationCount":"0","resultStr":"{\"title\":\"Isolation and molecular characterization of infectious bursal disease virus circulating in western and central India.\",\"authors\":\"A A Agnihotri, S P Awandkar, R C Kulkarni, S G Chavhan, R D Suryawanshi, A Mohan, M B Kulkarni, V G Chavan\",\"doi\":\"10.22099/IJVR.2023.47037.6774\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Infectious bursal disease (IBD) caused substantial economic loss in central and western India during 2020 and 2021.</p><p><strong>Aims: </strong>The study was conducted to characterize IBD virus (IBDV) from field outbreaks.</p><p><strong>Methods: </strong>The study was conducted on 360 samples from 42 poultry flocks. The samples were subjected to histopathology and molecular detection, followed by phylogenetic typing of the partial <i>VP2</i> gene.</p><p><strong>Results: </strong>The mortality ranged from 15.25 to 60.18%. The necropsy showed hemorrhages on thigh muscles, mottled spleen, swelling of kidneys and bursae with hemorrhages, and cheesy exudate. Histopathology revealed extensive necrosis and depletion of follicular lymphoid cells within the cortex and medulla, along with widespread hemorrhages, edema, and cystic cavities in the bursa. The field isolates showed cytopathic effects in the seventh passage. The cytopathic effects included swelling, rounding, granulation of cytoplasm around the nucleus, fragmentation of the infected cells, and detachment. The reverse transcription-polymerase chain reaction amplified 664 bp partial <i>VP2</i> gene. The phylogenetic analysis identified 19 field isolates as very virulent IBDVs (vvIBDVs) and three as classical strains.</p><p><strong>Conclusion: </strong>The results indicated that different vvIBDV strains are involved in disease outbreaks in central and western India.</p>\",\"PeriodicalId\":14629,\"journal\":{\"name\":\"Iranian journal of veterinary research\",\"volume\":\"24 4\",\"pages\":\"345-350\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11127732/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian journal of veterinary research\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.22099/IJVR.2023.47037.6774\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian journal of veterinary research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.22099/IJVR.2023.47037.6774","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Isolation and molecular characterization of infectious bursal disease virus circulating in western and central India.
Background: Infectious bursal disease (IBD) caused substantial economic loss in central and western India during 2020 and 2021.
Aims: The study was conducted to characterize IBD virus (IBDV) from field outbreaks.
Methods: The study was conducted on 360 samples from 42 poultry flocks. The samples were subjected to histopathology and molecular detection, followed by phylogenetic typing of the partial VP2 gene.
Results: The mortality ranged from 15.25 to 60.18%. The necropsy showed hemorrhages on thigh muscles, mottled spleen, swelling of kidneys and bursae with hemorrhages, and cheesy exudate. Histopathology revealed extensive necrosis and depletion of follicular lymphoid cells within the cortex and medulla, along with widespread hemorrhages, edema, and cystic cavities in the bursa. The field isolates showed cytopathic effects in the seventh passage. The cytopathic effects included swelling, rounding, granulation of cytoplasm around the nucleus, fragmentation of the infected cells, and detachment. The reverse transcription-polymerase chain reaction amplified 664 bp partial VP2 gene. The phylogenetic analysis identified 19 field isolates as very virulent IBDVs (vvIBDVs) and three as classical strains.
Conclusion: The results indicated that different vvIBDV strains are involved in disease outbreaks in central and western India.
期刊介绍：
The Iranian Journal of Veterinary Research(IJVR) is published quarterly in 4 issues. The aims of this journal are to improve and expand knowledge in all veterinary fields. It is an international journal indexed by the Thomson Institute for Scientific Information (ISI), Elsevier, Scopus, CAB International, Veterinary Bulletin and several other international databases. Research papers and reports on a wide range of veterinary topics are published in the journal after being evaluated by expert reviewers.The Editor-in-Chief is responsible for the editorial content of the journal—including peer-reviewed manuscripts—and the timing of its publication.
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