用于活细胞无标记成像的高分辨率相位对比显微系统

IF 2 4区生物学 Q4 CELL BIOLOGY

Cell structure and function Pub Date : 2024-05-25 DOI:10.1247/csf.24018

Kentaro Shimasaki, Yuko Okemoto-Nakamura, Saito Kyoko, Masayoshi Fukasawa, Kaoru Katoh, Kentaro Hanada

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引用次数: 0

摘要

长期以来，细胞生物学家一直在寻求在不使用标签的情况下观察活细胞内结构的能力。本研究介绍了如何调整市售的光栅相位对比（APC）显微镜系统，以更好地观察活细胞中各种亚细胞器的动态行为。利用这种技术捕捉连续图像的多功能性，我们可以实时观察病毒感染后细胞几何形态的变化，而无需探针或侵入性染色。调整型 APC 显微镜系统是一种高效平台，可同时观察不同亚细胞结构的动态行为，并具有极高的分辨率。

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A high-resolution phase-contrast microscopy system for label-free imaging in living cells

Cell biologists have long sought the ability to observe intracellular structures in living cells without labels. This study presents procedures to adjust a commercially available apodized phase-contrast (APC) microscopy system for better visualizing the dynamic behaviors of various subcellular organelles in living cells. By harnessing the versatility of this technique to capture sequential images, we could observe morphological changes in cellular geometry after virus infection in real time without probes or invasive staining. The tune-up APC microscopy system is a highly efficient platform for simultaneously observing the dynamic behaviors of diverse subcellular structures with exceptional resolution.

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来源期刊

Cell structure and function 生物-细胞生物学

CiteScore

2.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Cell Structure and Function is a fully peer-reviewed, fully Open Access journal. As the official English-language journal of the Japan Society for Cell Biology, it is published continuously online and biannually in print. Cell Structure and Function publishes important, original contributions in all areas of molecular and cell biology. The journal welcomes the submission of manuscripts on research areas such as the cell nucleus, chromosomes, and gene expression; the cytoskeleton and cell motility; cell adhesion and the extracellular matrix; cell growth, differentiation and death; signal transduction; the protein life cycle; membrane traffic; and organelles.