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引用次数: 0
摘要
本研究在纳米孔平台上开发了一种新型的铁(II)检测方法。哑铃状 DNA 和博来霉素（Bleomycin，BLM）的混合培养液会在纳米孔传感器上产生阻塞信号。加入铁（II）会生成 BLM-Fe（II），破坏哑铃 DNA 分子，释放出短链寡核苷酸片段，从而在纳米孔传感器上产生尖峰电流信号。统计分析显示，随着铁（II）浓度的升高，相邻阻塞信号之间的时间间隔会缩短，在 0.1 至 500 nM 的范围内，事件时间间隔与铁（II）浓度之间呈线性关系，检测限低至 0.12 nM。对各种金属离子进行了比较，结果表明对铁（II）的检测具有很高的选择性。该方法灵敏度高、选择性强，可用于铁(II)的定性和定量检测。
A Novel Detection Method of Fe(II) Based on Nanopore Sensor
A novel Fe(II) detection method was developed on nanopore platform in this work. The incubation mixture of dumbbell DNA and Bleomycin (BLM) wounld produce blockage signals on nanopore sensor. The addition of Fe(II) would generate BLM·Fe(II) to destroy the dumbbell DNA molecules and release short-strand oligonucleotide fragments which can produce spiked current signals on nanopore sensor. The statistical analysis shows the intervals between adjacent blockage signals decrease with elevated concentration of Fe(II), and a linear relationship between the event intervals versus the Fe(II) concentration is confirmed in the range of 0.1 to 500 nM with detection limit as low as 0.12 nM. Varied kinds of metal ions were examined for comparison, demonstrating high selectivity for Fe(II) detection. The proposed method may be potentially applied for qualitative and quantitative detection of Fe(II) due to its high sensitivity and selectivity.
期刊介绍：
Russian Journal of Electrochemistry is a journal that covers all aspects of research in modern electrochemistry. The journal welcomes submissions in English or Russian regardless of country and nationality of authors.
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