{"title":"河北省家禽耐多药大肠埃希菌mcr-1编码菌株的流行动态和遗传特征","authors":"","doi":"10.1016/j.jgar.2024.04.001","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><p>Colistin is known as the last resort antibiotic to treat the infections caused by multidrug resistant foodborne pathogens. The emergence and widespread dissemination of plasmid-mediated colistin resistance gene <em>mcr-1</em> in the <em>Escherichia coli</em> (<em>E. coli</em>) incurs potential threat to public health. Here, we investigated the epidemiology, transmission dynamics, and genetic characterization of <em>mcr-1</em> harbouring <em>E. coli</em> isolates from poultry originated in Hebei Province, China.</p></div><div><h3>Methods</h3><p>A total of 297 faecal samples were collected from the two large poultry farms in Hebei Province, China. The samples were processed for <em>E. coli</em> identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry and 16S rDNA sequencing. Then, the <em>mcr-1</em> gene harbouring <em>E. coli</em> strains were identified by polymerase chain reaction and subjected to antimicrobial susceptibility testing by broth microdilution assay. The genomic characterization of the isolates was done by whole genome sequencing using the various bioinformatics tools, and multi-locus sequence typing was done by sequence analysis of the seven housekeeping genes. The conjugation experiment was done to check the transferability of <em>mcr-1</em> along with the plasmid stability testing.</p></div><div><h3>Results</h3><p>A total of six <em>mcr-1 E. coli</em> isolates with minimum inhibitory concentration of 4 μg/mL were identified from 297 samples (2.02%). The <em>mcr-1</em> harbouring <em>E. coli</em> were identified as multidrug resistant and belonged to ST101 (<em>n</em> = 4) and ST410 (<em>n</em> = 2). The genetic environment of <em>mcr-1</em> presented its position on IncHI2 plasmid in 4 isolates and p0111 in 2 isolates, which is a rarely reported plasmid type for <em>mcr-1</em>. Moreover, both type of plasmids was transferable to recipient J53, and <em>mcr-1</em> was flanked by 3 mobile elements <em>ISApl1</em>, Tn3, and IS26 forming a novel backbone <em>Tn3–IS26-mcr-1– pap2-ISApl1</em> on the p0111 plasmid. The phylogenetic analysis shared a common lineage with <em>mcr-1</em> harbouring isolates from the environment, humans, and animals, which indicate its horizontal spread among the diverse sources, species, and hosts.</p></div><div><h3>Conclusion</h3><p>This study recommends the one health approach for future surveillance across multiple sources and bacterial species to adopt relevant measures and reduce global resistance crises.</p></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213716524000717/pdfft?md5=72df3281fa9ca548cffee5394952fab4&pid=1-s2.0-S2213716524000717-main.pdf","citationCount":"0","resultStr":"{\"title\":\"The prevalent dynamic and genetic characterization of mcr-1 encoding multidrug resistant Escherichia coli strains recovered from poultry in Hebei, China\",\"authors\":\"\",\"doi\":\"10.1016/j.jgar.2024.04.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objectives</h3><p>Colistin is known as the last resort antibiotic to treat the infections caused by multidrug resistant foodborne pathogens. The emergence and widespread dissemination of plasmid-mediated colistin resistance gene <em>mcr-1</em> in the <em>Escherichia coli</em> (<em>E. coli</em>) incurs potential threat to public health. Here, we investigated the epidemiology, transmission dynamics, and genetic characterization of <em>mcr-1</em> harbouring <em>E. coli</em> isolates from poultry originated in Hebei Province, China.</p></div><div><h3>Methods</h3><p>A total of 297 faecal samples were collected from the two large poultry farms in Hebei Province, China. The samples were processed for <em>E. coli</em> identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry and 16S rDNA sequencing. Then, the <em>mcr-1</em> gene harbouring <em>E. coli</em> strains were identified by polymerase chain reaction and subjected to antimicrobial susceptibility testing by broth microdilution assay. The genomic characterization of the isolates was done by whole genome sequencing using the various bioinformatics tools, and multi-locus sequence typing was done by sequence analysis of the seven housekeeping genes. The conjugation experiment was done to check the transferability of <em>mcr-1</em> along with the plasmid stability testing.</p></div><div><h3>Results</h3><p>A total of six <em>mcr-1 E. coli</em> isolates with minimum inhibitory concentration of 4 μg/mL were identified from 297 samples (2.02%). The <em>mcr-1</em> harbouring <em>E. coli</em> were identified as multidrug resistant and belonged to ST101 (<em>n</em> = 4) and ST410 (<em>n</em> = 2). The genetic environment of <em>mcr-1</em> presented its position on IncHI2 plasmid in 4 isolates and p0111 in 2 isolates, which is a rarely reported plasmid type for <em>mcr-1</em>. Moreover, both type of plasmids was transferable to recipient J53, and <em>mcr-1</em> was flanked by 3 mobile elements <em>ISApl1</em>, Tn3, and IS26 forming a novel backbone <em>Tn3–IS26-mcr-1– pap2-ISApl1</em> on the p0111 plasmid. The phylogenetic analysis shared a common lineage with <em>mcr-1</em> harbouring isolates from the environment, humans, and animals, which indicate its horizontal spread among the diverse sources, species, and hosts.</p></div><div><h3>Conclusion</h3><p>This study recommends the one health approach for future surveillance across multiple sources and bacterial species to adopt relevant measures and reduce global resistance crises.</p></div>\",\"PeriodicalId\":15936,\"journal\":{\"name\":\"Journal of global antimicrobial resistance\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-05-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2213716524000717/pdfft?md5=72df3281fa9ca548cffee5394952fab4&pid=1-s2.0-S2213716524000717-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of global antimicrobial resistance\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2213716524000717\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of global antimicrobial resistance","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213716524000717","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
The prevalent dynamic and genetic characterization of mcr-1 encoding multidrug resistant Escherichia coli strains recovered from poultry in Hebei, China
Objectives
Colistin is known as the last resort antibiotic to treat the infections caused by multidrug resistant foodborne pathogens. The emergence and widespread dissemination of plasmid-mediated colistin resistance gene mcr-1 in the Escherichia coli (E. coli) incurs potential threat to public health. Here, we investigated the epidemiology, transmission dynamics, and genetic characterization of mcr-1 harbouring E. coli isolates from poultry originated in Hebei Province, China.
Methods
A total of 297 faecal samples were collected from the two large poultry farms in Hebei Province, China. The samples were processed for E. coli identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry and 16S rDNA sequencing. Then, the mcr-1 gene harbouring E. coli strains were identified by polymerase chain reaction and subjected to antimicrobial susceptibility testing by broth microdilution assay. The genomic characterization of the isolates was done by whole genome sequencing using the various bioinformatics tools, and multi-locus sequence typing was done by sequence analysis of the seven housekeeping genes. The conjugation experiment was done to check the transferability of mcr-1 along with the plasmid stability testing.
Results
A total of six mcr-1 E. coli isolates with minimum inhibitory concentration of 4 μg/mL were identified from 297 samples (2.02%). The mcr-1 harbouring E. coli were identified as multidrug resistant and belonged to ST101 (n = 4) and ST410 (n = 2). The genetic environment of mcr-1 presented its position on IncHI2 plasmid in 4 isolates and p0111 in 2 isolates, which is a rarely reported plasmid type for mcr-1. Moreover, both type of plasmids was transferable to recipient J53, and mcr-1 was flanked by 3 mobile elements ISApl1, Tn3, and IS26 forming a novel backbone Tn3–IS26-mcr-1– pap2-ISApl1 on the p0111 plasmid. The phylogenetic analysis shared a common lineage with mcr-1 harbouring isolates from the environment, humans, and animals, which indicate its horizontal spread among the diverse sources, species, and hosts.
Conclusion
This study recommends the one health approach for future surveillance across multiple sources and bacterial species to adopt relevant measures and reduce global resistance crises.
期刊介绍:
The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes.
JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR).
Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.