河北省家禽耐多药大肠埃希菌mcr-1编码菌株的流行动态和遗传特征

IF 3.7 3区 医学 Q2 INFECTIOUS DISEASES
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引用次数: 0

摘要

目的:众所周知,可乐定是治疗由具有多重耐药性的食源性病原体引起的感染的最后一种抗生素。质粒介导的大肠杆菌耐药基因 mcr-1 的出现和广泛传播对公共卫生构成了潜在威胁。在此,我们研究了中国河北省家禽中携带 mcr-1 的大肠埃希氏菌分离物的流行病学、传播动态和遗传特征。样品经基质辅助激光解吸电离飞行时间质谱和 16S rDNA 测序鉴定。然后,通过聚合酶链式反应对含有 mcr-1 基因的大肠杆菌菌株进行鉴定,并采用肉汤微量稀释法进行抗菌药物敏感性试验。利用各种生物信息学工具对分离菌株进行了全基因组测序,并通过对七个看家基因的序列分析进行了多焦点序列分型。从 297 个样本(2.02%)中共鉴定出 6 个 mcr-1 大肠杆菌分离物,其最低抑菌浓度为 4 μg/mL。经鉴定,携带 mcr-1 的大肠杆菌具有多重耐药性,属于 ST101(4 个)和 ST410(2 个)。在 4 个分离株中,mcr-1 的基因环境位于 IncHI2 质粒上,在 2 个分离株中,mcr-1 的基因环境位于 p0111 质粒上。此外,这两种类型的质粒都能转移到受体 J53 上,mcr-1 的两侧有 ISApl1、Tn3 和 IS26 3 个移动元件,在 p0111 质粒上形成了一个新的骨架 Tn3-IS26-mcr-1-pap2-ISApl1。系统发生学分析表明,来自环境、人类和动物的 mcr-1 分离物具有共同的谱系,这表明它在不同来源、物种和宿主之间横向传播。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The prevalent dynamic and genetic characterization of mcr-1 encoding multidrug resistant Escherichia coli strains recovered from poultry in Hebei, China

Objectives

Colistin is known as the last resort antibiotic to treat the infections caused by multidrug resistant foodborne pathogens. The emergence and widespread dissemination of plasmid-mediated colistin resistance gene mcr-1 in the Escherichia coli (E. coli) incurs potential threat to public health. Here, we investigated the epidemiology, transmission dynamics, and genetic characterization of mcr-1 harbouring E. coli isolates from poultry originated in Hebei Province, China.

Methods

A total of 297 faecal samples were collected from the two large poultry farms in Hebei Province, China. The samples were processed for E. coli identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry and 16S rDNA sequencing. Then, the mcr-1 gene harbouring E. coli strains were identified by polymerase chain reaction and subjected to antimicrobial susceptibility testing by broth microdilution assay. The genomic characterization of the isolates was done by whole genome sequencing using the various bioinformatics tools, and multi-locus sequence typing was done by sequence analysis of the seven housekeeping genes. The conjugation experiment was done to check the transferability of mcr-1 along with the plasmid stability testing.

Results

A total of six mcr-1 E. coli isolates with minimum inhibitory concentration of 4 μg/mL were identified from 297 samples (2.02%). The mcr-1 harbouring E. coli were identified as multidrug resistant and belonged to ST101 (n = 4) and ST410 (n = 2). The genetic environment of mcr-1 presented its position on IncHI2 plasmid in 4 isolates and p0111 in 2 isolates, which is a rarely reported plasmid type for mcr-1. Moreover, both type of plasmids was transferable to recipient J53, and mcr-1 was flanked by 3 mobile elements ISApl1, Tn3, and IS26 forming a novel backbone Tn3–IS26-mcr-1– pap2-ISApl1 on the p0111 plasmid. The phylogenetic analysis shared a common lineage with mcr-1 harbouring isolates from the environment, humans, and animals, which indicate its horizontal spread among the diverse sources, species, and hosts.

Conclusion

This study recommends the one health approach for future surveillance across multiple sources and bacterial species to adopt relevant measures and reduce global resistance crises.

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来源期刊
Journal of global antimicrobial resistance
Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY
CiteScore
8.70
自引率
2.20%
发文量
285
审稿时长
34 weeks
期刊介绍: The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
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