日本和印度尼西亚肝细胞癌临床病理亚组的免疫组化比较分析

IF 3.3 Q2 GASTROENTEROLOGY & HEPATOLOGY
Kathryn Effendi , Nur Rahadiani , Marini Stephanie , Yutaka Kurebayashi , Hanako Tsujikawa , Chyntia O.M. Jasirwan , Ridho A. Syaiful , Michiie Sakamoto
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引用次数: 0

摘要

背景基于免疫组化(IHC)分析的标准化病理评估可改善全球肝细胞癌(HCC)的诊断。我们评估了来自日本东京和印度尼西亚雅加达两所学术机构的 HCC 临床病理亚组的差异。对胆道/干细胞(B/S)标记物(细胞角蛋白19、类盐蛋白4、上皮细胞粘附分子)和Wnt/β-catenin(W/B)信号相关分子(β-catenin、谷氨酰胺合成酶)的IHC分析可确定基于IHC的亚组。在免疫亚型分类方面,CD3/CD79α双重免疫组化可评估T细胞和B细胞的浸润情况。结果印尼的 HCC 患者大多为 60 岁(66%),有乙型肝炎病毒(HBV)背景(82%),与日本的 HCC 患者(分别为 8%和 19%,均为 P<0.001)形成鲜明对比。与日本病例相比,印尼病例更常见于肿瘤大小为5厘米(74% vs 23%,P = 0.001)、分化差(40% vs 24%)、门静脉侵犯(80% vs 61%)和α-胎儿蛋白水平为500纳克/毫升(45% vs 13%,P = 0.005)。两国 B/S、W/B 和 -/- 亚组的比例无明显差异。印尼病例中未发现免疫高肿瘤,免疫低肿瘤(66%)比日本病例(54%)更常见。印尼的 VETC 阳性肿瘤明显更常见(29%),且大多数属于 HBV(90%)和 -/- 亚组(90%),而日本的 VETC 病例(10%,P = 0.030)属于非病毒(100%)和 W/B 亚组(75%)。这些发现为各国的标准化尝试和 HCC 异质性提供了新的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative Immunohistochemical Analysis of Clinicopathological Subgroups in Hepatocellular Carcinomas from Japan and Indonesia

Background

Standardized pathological evaluation based on immunohistochemical (IHC) analysis could improve hepatocellular carcinoma (HCC) diagnoses worldwide. We evaluated differences in clinicopathological subgroups in HCCs from two academic institutions in Tokyo-Japan, and Jakarta-Indonesia.

Methods

Clinicopathological parameters and molecular expression patterns were evaluated in 35 HCCs from Indonesia and 41 HCCs from Japan. IHC analysis of biliary/stem cell (B/S) markers (cytokeratin 19, sal-like protein 4, epithelial cell adhesion molecule) and Wnt/β-catenin (W/B) signaling-related molecules (β-catenin, glutamine synthetase) could determine the IHC-based subgroups. For immuno-subtypes categorization, CD3/CD79α double immunohistochemistry was done to evaluate the infiltration of T and B cells. CD34 staining allowed identification of vessels that encapsulated tumor clusters (VETC).

Results

Indonesian HCC patients were mostly <60 years old (66%) with a hepatitis B virus (HBV) background (82%), in contrast to Japanese HCC patients (8% and 19%, respectively, both P < 0.001). In comparison with Japanese, Indonesian cases more frequently had >5 cm tumor size (74% vs 23%, P = 0.001), poor differentiation (40% vs 24%), portal vein invasion (80% vs 61%), and α-fetoprotein levels >500 ng/ml (45% vs 13%, P = 0.005). No significant differences were found in the proportions of B/S, W/B, and −/− subgroups from both countries. No immune-high tumors were observed among Indonesian cases, and immune-low tumors (66%) were more common than in Japanese cases (54%). VETC-positive tumors in Indonesia were significantly more common (29%), and most were in the HBV (90%) and −/− subgroups (90%), whereas Japanese VETC cases (10%, P = 0.030) were nonviral (100%) and W/B subgroups (75%).

Conclusion

IHC-based analysis more precisely reflected the clinicopathological differences of HCCs in Japan and Indonesia. These findings provide new insights into standardization attempts and HCC heterogeneity among countries.

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来源期刊
Journal of Clinical and Experimental Hepatology
Journal of Clinical and Experimental Hepatology GASTROENTEROLOGY & HEPATOLOGY-
CiteScore
4.90
自引率
16.70%
发文量
537
审稿时长
64 days
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