{"title":"一步式反转录数字 PCR 检测诺如病毒 GI 的验证","authors":"Bomin Ko , Taejin Shin , Boram Kim , Da-Hye Lee","doi":"10.1016/j.ab.2024.115576","DOIUrl":null,"url":null,"abstract":"<div><p>Regular monitoring of Norovirus presence in environmental and food samples is crucial due to its high transmission rates and outbreak potential. For detecting Norovirus GI, reverse transcription qPCR method is commonly used, but its sensitivity can be affected by assay performance. This study shows significantly reduced assay performance in digital PCR or qPCR when using primers targeting Norovirus GI genome 5291–5319 (NC_001959), located on the hairpin of the predicted RNA structure. It is highly recommended to avoid this region in commercial kit development or diagnosis to minimizing potential risk of false negatives.</p></div>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0003269724001209/pdfft?md5=79f03c6676d3608fb16a89de6bab090d&pid=1-s2.0-S0003269724001209-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Validation of one-step reverse transcription digital PCR assays for Norovirus GI\",\"authors\":\"Bomin Ko , Taejin Shin , Boram Kim , Da-Hye Lee\",\"doi\":\"10.1016/j.ab.2024.115576\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Regular monitoring of Norovirus presence in environmental and food samples is crucial due to its high transmission rates and outbreak potential. For detecting Norovirus GI, reverse transcription qPCR method is commonly used, but its sensitivity can be affected by assay performance. This study shows significantly reduced assay performance in digital PCR or qPCR when using primers targeting Norovirus GI genome 5291–5319 (NC_001959), located on the hairpin of the predicted RNA structure. It is highly recommended to avoid this region in commercial kit development or diagnosis to minimizing potential risk of false negatives.</p></div>\",\"PeriodicalId\":2,\"journal\":{\"name\":\"ACS Applied Bio Materials\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2024-05-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0003269724001209/pdfft?md5=79f03c6676d3608fb16a89de6bab090d&pid=1-s2.0-S0003269724001209-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Bio Materials\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0003269724001209\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0003269724001209","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
引用次数: 0
摘要
由于诺罗病毒的传播率高，有可能爆发疫情，因此定期监测环境和食物样本中是否存在诺罗病毒至关重要。检测诺罗病毒 GI 通常使用反转录 qPCR 方法，但其灵敏度会受到检测性能的影响。本研究表明，当使用针对诺罗病毒 GI 基因组 5291-5319 (NC_001959)（位于预测 RNA 结构的发夹）的引物时，数字 PCR 或 qPCR 的检测性能会明显降低。强烈建议在商业试剂盒开发或诊断中避免使用该区域，以最大限度地降低假阴性的潜在风险。
Validation of one-step reverse transcription digital PCR assays for Norovirus GI
Regular monitoring of Norovirus presence in environmental and food samples is crucial due to its high transmission rates and outbreak potential. For detecting Norovirus GI, reverse transcription qPCR method is commonly used, but its sensitivity can be affected by assay performance. This study shows significantly reduced assay performance in digital PCR or qPCR when using primers targeting Norovirus GI genome 5291–5319 (NC_001959), located on the hairpin of the predicted RNA structure. It is highly recommended to avoid this region in commercial kit development or diagnosis to minimizing potential risk of false negatives.
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