CD40 刺激可激活 CD8+ T 细胞并控制 CD4 缺失小鼠的 HBV

IF 9.5 1区 医学 Q1 GASTROENTEROLOGY & HEPATOLOGY
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引用次数: 0

摘要

背景& 目的由于共价闭合环状 DNA 复制池持续存在,且不受抗病毒干预的影响,因此 HBV 治疗具有挑战性。方法用编码 HBV 基因组的腺相关病毒(AAV-HBV)转导小鼠以启动 HBV 复制,并给小鼠注射激动剂 CD40 抗体。除 CD40 抗体外,还注射了 CD4 清除抗体。随时间推移测量血液中的病毒抗原,以确定 HBV 控制情况。结果CD40刺激CD4-depleted AAV-HBV小鼠可清除HBsAg和HBeAg,同时肝脏HBV mRNA减少,这与CD4-competent小鼠形成鲜明对比。CD8+T细胞是CD40介导的HBV控制不可或缺的细胞,这是由CD8+T细胞耗竭后HBV持续存在所决定的。在CD4缺失的小鼠中,CD40刺激最初促进了HBV特异性CD8+T细胞的扩增,但这些细胞随后无法控制HBV。最后,α-CD4/CD40 治疗降低了慢性 AAV-HBV 小鼠的抗原血症和肝脏 HBV mRNA 水平,通过与 VSV-MHBs(表达中型 HBsAg 的水泡性口炎病毒)免疫的协同作用进一步提高了抗原血症和肝脏 HBV mRNA 水平。影响和意义:免疫疗法有可能克服慢性 HBV 感染中的免疫功能障碍。这项研究利用小鼠 HBV 复制模型表明,CD40 刺激可诱导持续的 HBV 控制,这种控制依赖于 CD8+ T 细胞,并通过联合免疫进一步增强。出乎意料的是,CD40 介导的 HBV 减少可通过消耗 CD4+ 细胞得到改善。这些发现为逆转感染者体内的 HBV 持续存在提出了潜在的策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

CD40 stimulation activates CD8+ T cells and controls HBV in CD4-depleted mice

CD40 stimulation activates CD8+ T cells and controls HBV in CD4-depleted mice

Background & Aims

HBV treatment is challenging due to the persistence of the covalently closed circular DNA replication pool, which remains unaffected by antiviral intervention. In this study, we determined whether targeting antigen-presenting cells via CD40 stimulation represents an appropriate therapeutic approach for achieving sustained HBV control in a mouse model of HBV replication.

Methods

Mice were transduced with an adeno-associated virus encoding the HBV genome (AAV-HBV) to initiate HBV replication and were administered agonistic CD40 antibody. CD4-depleting antibody was administered in addition to the CD40 antibody. Viral antigens in the blood were measured over time to determine HBV control. HBV-specific CD8+ T cells were quantified in the spleen and liver at the experimental endpoint.

Results

CD40 stimulation in CD4-depleted AAV-HBV mice resulted in the clearance of HBsAg and HBeAg, along with a reduction in liver HBV mRNA, contrasting with CD4-competent counterparts. CD8+ T cells were indispensable for CD40-mediated HBV control, determined by HBV persistence following their depletion. In CD4-replete mice, CD40 stimulation initially facilitated the expansion of HBV-specific CD8+ T cells, which subsequently could not control HBV. Finally, α-CD4/CD40 treatment reduced antigenemia and liver HBV mRNA levels in chronic AAV-HBV mice, with further enhancement through synergy with immunization by VSV-MHBs (vesicular stomatitis virus expressing middle HBsAg).

Conclusions

Our findings underscore the potential of CD40 stimulation as a targeted therapeutic strategy for achieving sustained HBV control and reveal a CD4+ T cell-dependent limitation on CD40-mediated antiviral efficacy.

Impact and implications:

Immunotherapy has the potential to overcome immune dysfunction in chronic HBV infection. Using a mouse model of HBV replication, this study shows that CD40 stimulation can induce sustained HBV control, which is dependent on CD8+ T cells and further enhanced by co-immunization. Unexpectedly, CD40-mediated HBV reduction was improved by the depletion of CD4+ cells. These findings suggest potential strategies for reversing HBV persistence in infected individuals.

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来源期刊
JHEP Reports
JHEP Reports GASTROENTEROLOGY & HEPATOLOGY-
CiteScore
12.40
自引率
2.40%
发文量
161
审稿时长
36 days
期刊介绍: JHEP Reports is an open access journal that is affiliated with the European Association for the Study of the Liver (EASL). It serves as a companion journal to the highly respected Journal of Hepatology. The primary objective of JHEP Reports is to publish original papers and reviews that contribute to the advancement of knowledge in the field of liver diseases. The journal covers a wide range of topics, including basic, translational, and clinical research. It also focuses on global issues in hepatology, with particular emphasis on areas such as clinical trials, novel diagnostics, precision medicine and therapeutics, cancer research, cellular and molecular studies, artificial intelligence, microbiome research, epidemiology, and cutting-edge technologies. In summary, JHEP Reports is dedicated to promoting scientific discoveries and innovations in liver diseases through the publication of high-quality research papers and reviews covering various aspects of hepatology.
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