Aasma Hashmi, Hamd Syed, Ayesha Irfan, Ali Rabbani, Amaidah Mir
{"title":"量化肾脏变化:白化大鼠长期接触盐酸伊托必利后鲍曼间隙面积的研究","authors":"Aasma Hashmi, Hamd Syed, Ayesha Irfan, Ali Rabbani, Amaidah Mir","doi":"10.37185/lns.1.1.539","DOIUrl":null,"url":null,"abstract":"Objective: To see the effects of long-term administration of itopride hydrochloride on the histomorphology of kidney of the male Wistar albino rats.Study Design: A Laboratory based experimental study.Place and Duration of Study: The study was conducted at the Department of Anatomy, CMH Multan Institute of Medical Sciences Multan, Pakistan from September 2020 to March 2022.Methods: The study involved two groups: Group A served as the control, and Group B functioned as the experimental group. Each group consisted of 30 adult male albino rats. The animals of group A were administered 2ml/100g of distilled water by oral gavage along with a diet for laboratory rodents daily for 15 days. The animals of group B were given 4.4mg/kg of itopride hydrochloride dissolved in 2ml/100g body weight of distilled water by oral gavage three times a day for 15 days. The kidney was processed for paraffin embedding and stained with Periodic Acid Schiff (PAS). The area of Bowman's space was measured by linear micrometer after calibration with a stage micrometer and image J software. The student's t-test was utilized to assess potential differences between the two groups, with significance set at a p-value of ≤ 0.05.Result: The area of Bowman's space was significantly increased in the experimental group as compared to the control group (p= 0.045).Conclusion: Long-term Itopride Hydrochloride administration appears to influence the area of Bowman's Space in Wistar albino rats. \nHow to cite this: Hashmi A, Syed H, Irfan A, Rabbani A, Mir A, Razzaq M. Quantifying Renal Changes: A Study of Area of Bowman's Space Following Prolonged Itopride Hydrochloride Exposure in Albino Rats. Life and Science. 2024; 5(2): 138-143. doi: http://doi.org/10.37185/LnS.1.1.539","PeriodicalId":516717,"journal":{"name":"Life and Science","volume":" 48","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Quantifying Renal Changes: A Study of Area of Bowman's Space Following Prolonged Itopride Hydrochloride Exposure in Albino Rats\",\"authors\":\"Aasma Hashmi, Hamd Syed, Ayesha Irfan, Ali Rabbani, Amaidah Mir\",\"doi\":\"10.37185/lns.1.1.539\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective: To see the effects of long-term administration of itopride hydrochloride on the histomorphology of kidney of the male Wistar albino rats.Study Design: A Laboratory based experimental study.Place and Duration of Study: The study was conducted at the Department of Anatomy, CMH Multan Institute of Medical Sciences Multan, Pakistan from September 2020 to March 2022.Methods: The study involved two groups: Group A served as the control, and Group B functioned as the experimental group. Each group consisted of 30 adult male albino rats. The animals of group A were administered 2ml/100g of distilled water by oral gavage along with a diet for laboratory rodents daily for 15 days. The animals of group B were given 4.4mg/kg of itopride hydrochloride dissolved in 2ml/100g body weight of distilled water by oral gavage three times a day for 15 days. The kidney was processed for paraffin embedding and stained with Periodic Acid Schiff (PAS). The area of Bowman's space was measured by linear micrometer after calibration with a stage micrometer and image J software. The student's t-test was utilized to assess potential differences between the two groups, with significance set at a p-value of ≤ 0.05.Result: The area of Bowman's space was significantly increased in the experimental group as compared to the control group (p= 0.045).Conclusion: Long-term Itopride Hydrochloride administration appears to influence the area of Bowman's Space in Wistar albino rats. \\nHow to cite this: Hashmi A, Syed H, Irfan A, Rabbani A, Mir A, Razzaq M. Quantifying Renal Changes: A Study of Area of Bowman's Space Following Prolonged Itopride Hydrochloride Exposure in Albino Rats. 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引用次数: 0
摘要
研究目的观察长期服用盐酸伊托必利对雄性 Wistar 白化大鼠肾脏组织形态学的影响:实验室实验研究:研究于 2020 年 9 月至 2022 年 3 月在巴基斯坦木尔坦 CMH 医学院解剖学系进行:研究分为两组:A组为对照组,B组为实验组。每组由 30 只成年雄性白化大鼠组成。A 组动物每天口服 2 毫升/100 克蒸馏水和实验鼠饮食,连续 15 天。给 B 组大鼠口服溶于每 100 克体重 2 毫升蒸馏水中的 4.4 毫克/千克盐酸伊托必利,每天三次,连续 15 天。对肾脏进行石蜡包埋处理,并用过碘酸希夫(PAS)染色。用台式测微计和图像 J 软件校准后,用线性测微计测量鲍曼间隙的面积。采用学生 t 检验来评估两组之间的潜在差异,显著性以 p 值≤0.05 为限:结果:与对照组相比,实验组的鲍曼氏间隙面积明显增加(p= 0.045):结论:长期服用盐酸伊托必利可影响 Wistar 白化大鼠鲍曼氏间隙的面积。引用方式Hashmi A, Syed H, Irfan A, Rabbani A, Mir A, Razzaq M. Quantifying Renal Changes:白化大鼠长期接触盐酸伊托必利后鲍曼间隙面积的研究》。生命与科学》。2024; 5(2):138-143. doi: http://doi.org/10.37185/LnS.1.1.539
Quantifying Renal Changes: A Study of Area of Bowman's Space Following Prolonged Itopride Hydrochloride Exposure in Albino Rats
Objective: To see the effects of long-term administration of itopride hydrochloride on the histomorphology of kidney of the male Wistar albino rats.Study Design: A Laboratory based experimental study.Place and Duration of Study: The study was conducted at the Department of Anatomy, CMH Multan Institute of Medical Sciences Multan, Pakistan from September 2020 to March 2022.Methods: The study involved two groups: Group A served as the control, and Group B functioned as the experimental group. Each group consisted of 30 adult male albino rats. The animals of group A were administered 2ml/100g of distilled water by oral gavage along with a diet for laboratory rodents daily for 15 days. The animals of group B were given 4.4mg/kg of itopride hydrochloride dissolved in 2ml/100g body weight of distilled water by oral gavage three times a day for 15 days. The kidney was processed for paraffin embedding and stained with Periodic Acid Schiff (PAS). The area of Bowman's space was measured by linear micrometer after calibration with a stage micrometer and image J software. The student's t-test was utilized to assess potential differences between the two groups, with significance set at a p-value of ≤ 0.05.Result: The area of Bowman's space was significantly increased in the experimental group as compared to the control group (p= 0.045).Conclusion: Long-term Itopride Hydrochloride administration appears to influence the area of Bowman's Space in Wistar albino rats.
How to cite this: Hashmi A, Syed H, Irfan A, Rabbani A, Mir A, Razzaq M. Quantifying Renal Changes: A Study of Area of Bowman's Space Following Prolonged Itopride Hydrochloride Exposure in Albino Rats. Life and Science. 2024; 5(2): 138-143. doi: http://doi.org/10.37185/LnS.1.1.539