煤尘对人体肝脏组织自由基过程的适应性矫正(实验研究)

M. S. Bugaeva, O. Bondarev, L. G. Gorokhova, A. G. Zhukova, N. Mikhailova
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引用次数: 0

摘要

简介在长期接触煤岩粉尘的情况下,人体肝脏受到损害,导致其代谢、解毒、合成和其他功能严重受损。因此,寻找早期预防和纠正该器官病变的方法具有重要意义。本研究的目的是通过实验研究一种含有双氢槲皮素的复方药物对长期暴露于煤岩尘埃的实验鼠肝脏自由基过程活性的影响。材料和方法实验对象为体重 200-250 克的雄性白鼠。实验组包括在 1、3、6 和 9 周内每天在引物室吸入煤岩尘 4 小时的大鼠,以及在接触煤岩尘之前每天立即服用含有双氢槲皮素的复合药物的大鼠。测定了肝组织中自由基氧化和抗氧化防御酶(超氧化物歧化酶和过氧化氢酶)的活性。结果显示用煤岩粉尘对大鼠进行为期三周的预处理会导致肝组织中自由基过程的强度增加:与对照组相比,TBA活性氧化产物的初始积累水平在实验的第一周和第三周分别提高了近2倍和3倍。在自由基氧化强度增加的同时,抗氧化防御酶(过氧化氢酶)也被激活,在接触粉尘的第一周和第三周分别增加了 2.4 倍和 1.6 倍。将粉尘引诱期延长至 9 周会导致肝组织氧化代谢发生多向变化:第 6 周时,超氧化物歧化酶的活性降低了近 3 倍;第 9 周时,抗氧化酶略有激活:超氧化物歧化酶激活了 55%,过氧化氢酶激活了 36%。二氢槲皮素的最大抗氧化防御效果是在接触粉尘的第 3 周检测到的--自由基产物的水平降低了 2 倍,超氧化物歧化酶的活性提高了 4 倍。局限性。由于对双氢槲皮素对器官的特异性作用以及使用双氢槲皮素提高对自由基损伤的抵抗力的最佳持续时间等问题的研究还很少,这对使用双氢槲皮素治疗和预防职业病造成了一定的限制。结论在长期使用外源性抗氧化剂治疗和预防职业病时,应考虑到所获得的结果,以支持细胞中内源性抗氧化系统的水平,以及二氢槲皮素对最易受自由基作用的组织的保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Adaptogenic correction of free radical processes in liver tissue under the exposure to coal-rock dust on the body (experimental study)
Introduction. Damage to the liver under the conditions of long-term exposure to coal-rock dust on the body leads to significant impairment of its metabolic, detoxification, synthetic, and other functions. In this regard, it is relevant to search for early methods of prevention and correction of developing pathological changes in this organ. The purpose of the study is to experimentally study the effect of a complex drug with dihydroquercetin on the activity of free radical processes in the liver of laboratory rats over the dynamics of long-term exposure to coal-rock dust. Materials and methods. The work was carried out on male white rats weighing 200–250 g. The control group was kept under the standard vivarium conditions. The experimental group included rats that inhaled coal-rock dust in a priming chamber daily for 4 hours for 1, 3, 6, and 9 weeks and rats that received a daily complex drug with dihydroquercetin immediately before dust exposure. In the liver tissue, the activity of free radical oxidation and antioxidant defense enzymes (superoxide dismutase and catalase) was determined. Results. Priming rats with coal-rock dust for three weeks led to the gain in the intensity of free radical processes in the liver tissue: the initial level of accumulation of TBA-active oxidation products reliably raised by almost 2 times over the 1st week and 3 times over the 3rd week of the experiment compared to the control. An increase in the intensity of free radical oxidation was accompanied by activation of antioxidant defense enzymes - catalase - by 2.4 times at the 1st week and 1.6 times at the 3rd week of dust exposure. Raising the period of priming with dust up to 9 weeks led to multidirectional changes in oxidative metabolism in the liver tissue: at week 6, a decrease in the activity of superoxide dismutase was almost 3 times; at week 9, to a slight activation of antioxidant enzymes: superoxide dismutase by 55% and catalase by 36%.The most effective use of a complex drug with dihydroquercetin had been shown in short-term mode for three weeks, but not long-term (more than 6 weeks). The maximum antioxidant defense effect of dihydroquercetin was detected at the 3rd week of dust exposure – the level of free radical products decreased by 2 times and the activity of superoxide dismutase increased by 4 times. Limitations. Since the issue of organ-specific effects and the optimal duration of use of dihydroquercetin to increase resistance to free radical damage has been little elaborated, this imposes certain restrictions on its use for the correction and prevention of occupational diseases. Conclusion. The results obtained should be taken into account with long-term use of exogenous antioxidants in the correction and prevention of occupational diseases to support the level of endogenous antioxidant systems in the cell and the protective effect of dihydroquercetin for tissues most sensitive to the action of free radical processes.
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