用贯叶连翘毛根甲醇水提取物治疗糖尿病大鼠后对碳水化合物代谢和胰岛素分泌的调节作用

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-05-19 DOI:10.20450/mjcce.2024.2849

Elena Rafailovska, O. Tusevski, Sonja Gadzovska Simic, Sasho Panov, Jasmina Petreska Stanoeva, Suzana Dinevska- Kjovkarovska, B. Miova

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引用次数: 0

摘要

贯叶连翘的毛根（HR）培养物在控制高血糖、调节血脂和酶谱以及改善体内代谢功能方面显示出前景。这些益处主要归功于金丝桃提取物中的酚酸、类黄酮和黄酮。然而，这些作用的具体机制仍不清楚。本研究旨在阐明HR提取物抗高血糖和抗糖尿病作用的生化和分子机制。健康大鼠和链脲佐菌素诱导的糖尿病大鼠每天服用HR提取物（200毫克/千克体重）14天，以格列本脲作阳性对照。结果表明，HR 提取物能提高糖尿病大鼠的血浆胰岛素水平和胰腺多聚（二磷酸腺苷核糖）聚合酶（PARP）活性，从而使血糖水平恢复正常。此外，它还能降低葡萄糖生成酶的活性，提高糖酵解酶的活性，并使肝脏中的糖原含量恢复正常。总之，HR 提取物具有促胰岛素分泌作用，并能通过调节 AMPK 的表达和 PKCε 的浓度有效调节糖尿病大鼠肝脏的碳水化合物代谢。这些研究结果表明，HR 提取物可用作治疗糖尿病的中药，也可作为开发抗糖尿病药物的来源。

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Regulation of carbohydrate metabolism and insulin secretion in diabetic rats following treatment with Hypericum perforatum L. hairy root aqueous methanol extract

Hairy root (HR) cultures of Hypericum perforatum L. have shown promise in controlling hyperglycemia, regulating blood lipid and enzyme profiles, and improving metabolic function in vivo. These benefits are mainly attributed to the phenolic acids, flavonoids, and xanthones present in HR extracts. However, the specific mechanism underlying these effects remains unclear. This study was performed to elucidate the biochemical and molecular mechanisms driving HR the antihyperglycemic and antidiabetic effects of HR extracts. HR extract (200 mg/kg body weight) was administered daily for 14 days to healthy rats and rats with streptozotocin-induced diabetes, with glibenclamide serving as a positive control. The phenolic composition of the HR extracts was confirmed through high-performance liquid chromatography/diode array detection/electrospray ionization mass spectrometry (HPLC/DAD/ESI-MSn ) analysis. The results showed that HR extract treatment increased the plasma insulin level and pancreatic poly (adenosine diphosphate-ribose) polymerase (PARP) activity in diabetic rats, thus normalizing blood glucose levels. Additionally, it reduced the activity of gluconeogenic enzymes, increased the activity of glycolytic enzymes, and normalized the glycogen content in the liver. HR extract-treated rats also exhibited increased hepatic adenosine monophosphate-activated protein kinase (AMPK) mRNA expression and a decreased protein kinase Cε (PKCε) concentration. In conclusion, HR extract demonstrated insulinotropic effects and effectively regulated hepatic carbohydrate metabolism in diabetic rats by modulating AMPK expression and the PKCε concentration. These findings suggest the potential use of HR extract as an herbal medicine for diabetes treatment and a source of antidiabetic drug development.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.