诱导新生小鼠贫血和输注红细胞相关坏死性小肠结肠炎的详细方案

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-05-20 DOI:10.21769/BioProtoc.4993

Balamurugan Ramatchandirin, Marie Amalie Balamurugan, Suneetha Desiraju, Yerin Chung, K. Mohankumar

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引用次数: 0

摘要

贫血是一个常见而严重的健康问题，几乎所有早产儿都会患上贫血，而且贫血与全世界发病率和死亡率的增加有关。输注红细胞（RBC）是挽救生命的主要疗法，但也有严重的不良影响。其后果之一是早产儿坏死性小肠结肠炎（NEC），这是一种炎症性肠坏死疾病。通过建立抽血诱发贫血和输注红细胞相关坏死性小肠结肠炎的小鼠模型，可以对这些疾病的分子机制进行详细研究，并对潜在的新治疗策略进行评估。本方案描述了获得抽血诱发贫血的小鼠幼崽和输注发生 NEC 的 RBC 的详细过程。

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A Detailed Protocol for the Induction of Anemia and RBC Transfusion–associated Necrotizing Enterocolitis in Neonatal Mice

Anemia is a common and serious health problem, nearly universally diagnosed in preterm infants, and is associated with increased morbidity and mortality worldwide. Red blood cell (RBC) transfusion is a lifesaving and mainstay therapy; however, it has critical adverse effects. One consequence is necrotizing enterocolitis (NEC), an inflammatory bowel necrosis disease in preterm infants. The murine model of phlebotomy-induced anemia and RBC transfusion–associated NEC enables a detailed study of the molecular mechanisms underlying these morbidities and the evaluation of potential new therapeutic strategies. This protocol describes a detailed procedure for obtaining murine pups with phlebotomy-induced anemia and delivering an RBC transfusion that develops NEC.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.