对载入 IL-22 mRNA 的脂质纳米颗粒进行荧光标记和成像

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
R. J. Mow, Anand Srinivasan, Eunice Bolay, D. Merlin, Chunhua Yang
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引用次数: 0

摘要

基于脂质纳米颗粒(LNP)的给药系统(DDS)因其能够提高治疗剂(包括 DNA 和 mRNA 等核酸)的高效和精确给药而得到广泛认可。尽管如此,有关这些纳米颗粒的全身生物分布和器官蓄积的知识仍存在明显的空白。在体内跟踪 LNPs 的能力对于了解它们在生物系统中的命运至关重要。对 LNPs 进行荧光标记有助于对其在生物系统中的行为进行实时跟踪、量化和可视化,从而为生物分布、细胞摄取和优化给药策略提供有价值的见解。我们之前的研究证实,反向工程 LNPs 是治疗溃疡性结肠炎的一种特殊 mRNA 递送平台。本研究介绍了标记白细胞介素-22(IL-22)mRNA负载LNPs的详细方案、小鼠口服给药以及利用IVIS光谱观察DiR标记的LNPs在胃肠道中的生物分布。这种基于荧光的方法将有助于研究人员动态了解纳米粒子在其他相关模型中的命运。主要特点 - 本方案旨在评估利用脂质纳米颗粒向溃疡性结肠炎部位递送 IL-22 mRNA 的情况。- 本方案使用荧光 DiR 染料对小鼠胃肠道中装载 IL-22 mRNA 的脂质纳米颗粒进行成像。- 本方案采用 IVIS 光谱进行成像。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Fluorescent Labeling and Imaging of IL-22 mRNA-Loaded Lipid Nanoparticles
Lipid nanoparticle (LNP)-based drug delivery systems (DDSs) are widely recognized for their ability to enhance efficient and precise delivery of therapeutic agents, including nucleic acids like DNA and mRNA. Despite this acknowledgment, there is a notable knowledge gap regarding the systemic biodistribution and organ accumulation of these nanoparticles. The ability to track LNPs in vivo is crucial for understanding their fate within biological systems. Fluorescent labeling of LNPs facilitates real-time tracking, quantification, and visualization of their behavior within biological systems, providing valuable insights into biodistribution, cellular uptake, and the optimization of drug delivery strategies. Our prior research established reversely engineered LNPs as an exceptional mRNA delivery platform for treating ulcerative colitis. This study presents a detailed protocol for labeling interleukin-22 (IL-22) mRNA-loaded LNPs, their oral administration to mice, and visualization of DiR-labeled LNPs biodistribution in the gastrointestinal tract using IVIS spectrum. This fluorescence-based approach will assist researchers in gaining a dynamic understanding of nanoparticle fate in other models of interest. Key features • This protocol is developed to assess the delivery of IL-22 mRNA to ulcerative colitis sites using lipid nanoparticles. • This protocol uses fluorescent DiR dye for imaging of IL-22 mRNA-loaded lipid nanoparticles in the gastrointestinal tract of mice. • This protocol employs the IVIS spectrum for imaging.
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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