Qihui Wang, Ning Liu, Ruirui Yang, Xuejiao Zhang, Ying Wang, Yongcai Li, D. Prusky, Yang Bi, Ye Han
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引用次数: 0
摘要
脱落酸(ABA)是一种参与果蔬伤口愈合的关键植物激素,而氟啶酮(FLD)是其合成抑制剂。然而,ABA 信号和下游转录因子是否参与了麝香瓜伤口中酚酸和木质素单体的合成及其内在机制尚不清楚。在我们的研究中,外源ABA通过提高β-类胡萝卜素和玉米黄质的水平,激活9-顺式环氧类胡萝卜素二氧化酶(NCED)和玉米黄质环氧化酶(ZEP),促进内源ABA的合成;通过提高蛋白磷酸酶2C型(CmPP2C)和ABA反应元件结合因子(CmABF)的表达水平,促进ABA信号转导、ABA通过激活苯丙氨酸氨化裂解酶(PAL)、4-辅酶A连接酶(4CL)和肉桂醇脱氢酶(CAD)诱导苯丙氨酸代谢,进一步增加了麝香瓜伤口愈合过程中酚酸和木质素单体的合成。综上所述,外源 ABA 诱导了麝香瓜伤口愈合过程中的苯丙醇代谢,增加了酚酸和木质素单体的合成,可能与内源 ABA 合成、信号转导及相关转录因子有关。
Essential role of ABA signaling and related transcription factors in phenolic acid and lignin synthesis during muskmelon wound healing
Abscisic acid (ABA) is a key phytohormone involved in wound healing in fruits and vegetables, while fluridone (FLD) is its synthetic inhibitor. However, it is unknown whether ABA signaling and downstream transcription factors are involved in the synthesis of phenolic acids and lignin monomers in muskmelon wounds, and the underlying mechanisms. In our study, exogenous ABA promoted endogenous ABA synthesis by increasing the levels of β-carotenoid and zeaxanthin, activating 9-cis-epoxycarotenoid dioxygenase (NCED) and zeaxanthin epoxidase (ZEP), facilitated ABA signaling by increasing the expression levels of protein phosphatases type 2C (CmPP2C) and ABA-responsive element binding factors (CmABF), upregulated the expression levels of CmMYB1 and CmWRKY1, and ABA induced phenylpropanoid metabolism by activating phenylalanine ammonia-lyase (PAL), 4-coenzyme A ligase (4CL), and cinnamyl alcohol dehydrogenase (CAD), which further increased the synthesis of phenolic acids and lignin monomers in muskmelon wounds during healing. Taken together, exogenous ABA induced phenylpropanoid metabolism and increased the synthesis of phenolic acid and lignin monomer in muskmelon wounds during healing, and may be involved in endogenous ABA synthesis and signaling and related transcription factors.