昆虫载体 Anasa tristis 对共生体定殖的响应基因表达差异,而不是对媒介植物病原体感染的响应基因表达差异

IF 2.4 3区 环境科学与生态学 Q2 ECOLOGY
S. Mendiola, Jason Z. Chen, Ben Lukubye, D. Civitello, Nic M Vega, Nicole M. Gerardo
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引用次数: 0

摘要

许多昆虫会选择性地与特定微生物建立长期共生关系。维持这种关系对昆虫宿主的发展至关重要,但昆虫也必须应对环境中其他微生物可能带来的并发感染。在抵御微生物威胁的同时,许多昆虫发展出专门的共生器官来容纳有益微生物。共生体的建立虽然集中在这些器官的局部,但会对昆虫产生全球性影响,包括影响共感染微生物在昆虫宿主体内定殖的成功率。我们采用转录组学方法研究了农业害虫 Anasa tristis 与 Caballeronia 属细菌之间的互利共生如何影响昆虫共生器官局部和整个昆虫宿主的基因表达。同时,我们还确定 Caballeronia 的定殖是否会影响昆虫宿主对感染植物病原体 Serratia marcescens(它将病原体传播给植物)的反应。我们发现,感染 S. marcescens 不会引起明显的基因表达差异。这是一个令人惊讶的发现,因为以前的研究表明,与共生个体相比,共生的三疣梭菌能迅速清除 S. marcescens 感染。我们的研究结果表明,A. tristis 的共生组织和非共生组织在基因表达上存在很大差异,尤其是在共生体成功定殖之后。我们发现有证据表明,在共生器官内,宿主免疫力局部下调,细胞通讯功能上调,这些功能可促进三裂叶蓟马-Caballeronia共生的成功。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Differential gene expression in the insect vector Anasa tristis in response to symbiont colonization but not infection with a vectored phytopathogen
Many insects selectively associate with specific microbes in long-term, symbiotic relationships. Maintaining these associations can be vital for the insect hosts’ development, but insects must also contend with potential coinfections from other microbes in the environment. Fending off microbial threats while maintaining mutualistic microbes has resulted in many insects developing specialized symbiotic organs to house beneficial microbes. Though locally concentrated in these organs, symbiont establishment can have global consequences for the insect, including influence over the success of coinfecting microbes in colonizing the insect host. We use a transcriptomic approach to examine how the mutualistic symbiosis between the agricultural pest Anasa tristis and bacteria in the genus Caballeronia affects insect gene expression locally within the symbiotic organs and in the insect host at large. We simultaneously determine whether Caballeronia colonization impacts insect host responses to infection with the plant pathogen Serratia marcescens, which it vectors to plants. We found that no significant differential gene expression was elicited by infection with S. marcescens. This was a surprising finding given previous work indicating that symbiotic A. tristis clear S. marcescens infection rapidly compared to aposymbiotic individuals. Our results indicate that symbiotic and nonsymbiotic tissues in A. tristis differ greatly in their gene expression, particularly following successful symbiont colonization. We found evidence for local downregulation of host immunity and upregulation of cell communication within the symbiotic organs, functions which can facilitate the success of the A. tristis-Caballeronia symbiosis.
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来源期刊
Frontiers in Ecology and Evolution
Frontiers in Ecology and Evolution Environmental Science-Ecology
CiteScore
4.00
自引率
6.70%
发文量
1143
审稿时长
12 weeks
期刊介绍: Frontiers in Ecology and Evolution publishes rigorously peer-reviewed research across fundamental and applied sciences, to provide ecological and evolutionary insights into our natural and anthropogenic world, and how it should best be managed. Field Chief Editor Mark A. Elgar at the University of Melbourne is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics and the public worldwide. Eminent biologist and theist Theodosius Dobzhansky’s astute observation that “Nothing in biology makes sense except in the light of evolution” has arguably even broader relevance now than when it was first penned in The American Biology Teacher in 1973. One could similarly argue that not much in evolution makes sense without recourse to ecological concepts: understanding diversity — from microbial adaptations to species assemblages — requires insights from both ecological and evolutionary disciplines. Nowadays, technological developments from other fields allow us to address unprecedented ecological and evolutionary questions of astonishing detail, impressive breadth and compelling inference. The specialty sections of Frontiers in Ecology and Evolution will publish, under a single platform, contemporary, rigorous research, reviews, opinions, and commentaries that cover the spectrum of ecological and evolutionary inquiry, both fundamental and applied. Articles are peer-reviewed according to the Frontiers review guidelines, which evaluate manuscripts on objective editorial criteria. Through this unique, Frontiers platform for open-access publishing and research networking, Frontiers in Ecology and Evolution aims to provide colleagues and the broader community with ecological and evolutionary insights into our natural and anthropogenic world, and how it might best be managed.
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