Mahmoud Emam , Mona M.H. Soliman , Mohamed A. Tantawy , Mohamed A. El-Ansari , Mohamed Seif
{"title":"从印度芒果种仁提取物中提取五聚酰基葡萄糖和五倍子单宁作为有前景的抗真菌饲料添加剂的体外和硅学研究","authors":"Mahmoud Emam , Mona M.H. Soliman , Mohamed A. Tantawy , Mohamed A. El-Ansari , Mohamed Seif","doi":"10.1016/j.jspr.2024.102354","DOIUrl":null,"url":null,"abstract":"<div><p>Recently, the valorization of agro-industrial wastes through their exploration of their bioactive compounds has become a necessary trend to maximize their applications in various production sectors, particularly animal feeding. In the study, mango seed kernels were used as agro-industrial wastes to extract gallotannins fraction (GF) and pentagalloyl glucose (PGG). The GF and PGG were mixed with poultry feeds to investigate their potential ability to control <em>Aspergillus flavus</em> (<em>A. flavus</em>) growth and inhibit its production of aflatoxins (AFs). Ketoconazole was used as an antifungal standard. GF and PGG combat the growth of <em>A. flavus</em>, with minimum inhibition concentration (MIC) of 21.7 ± 1.7 and 20 ± 0 mg/mL, respectively. Moreover, GF and PGG showed promising results in preventing the production of AFs (AFB1, AFB2, AFG1, and AFG2) at 5 and 10 mg/100-g feed. Furthermore, to explain the mode of action of PGG as an antifungal agent, a molecular docking study has been performed against Type 1 Polyketide Synthase (PKS), which is found in many toxigenic <em>Aspergillus</em> species and has a highly active Acyl Carrier Protein (ACP) domain that is implicated in thiol ester-bound intermediates involved in fungal polyketides, fatty acids, and other non-ribosomal peptide formation, which are highly implicated in the construction of aflatoxin biosynthesis. The promising results prompt us to use mango seed extract as a cheap source of gallotannin components as a safe feed additive for reducing fungal and mycotoxin contamination in animal feeds.</p></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Pentagalloyl glucose and gallotannins from mangifera Indica L. seed kernel extract as a promising antifungal feed additive, in vitro, and in silico studies\",\"authors\":\"Mahmoud Emam , Mona M.H. Soliman , Mohamed A. Tantawy , Mohamed A. El-Ansari , Mohamed Seif\",\"doi\":\"10.1016/j.jspr.2024.102354\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Recently, the valorization of agro-industrial wastes through their exploration of their bioactive compounds has become a necessary trend to maximize their applications in various production sectors, particularly animal feeding. In the study, mango seed kernels were used as agro-industrial wastes to extract gallotannins fraction (GF) and pentagalloyl glucose (PGG). The GF and PGG were mixed with poultry feeds to investigate their potential ability to control <em>Aspergillus flavus</em> (<em>A. flavus</em>) growth and inhibit its production of aflatoxins (AFs). Ketoconazole was used as an antifungal standard. GF and PGG combat the growth of <em>A. flavus</em>, with minimum inhibition concentration (MIC) of 21.7 ± 1.7 and 20 ± 0 mg/mL, respectively. Moreover, GF and PGG showed promising results in preventing the production of AFs (AFB1, AFB2, AFG1, and AFG2) at 5 and 10 mg/100-g feed. Furthermore, to explain the mode of action of PGG as an antifungal agent, a molecular docking study has been performed against Type 1 Polyketide Synthase (PKS), which is found in many toxigenic <em>Aspergillus</em> species and has a highly active Acyl Carrier Protein (ACP) domain that is implicated in thiol ester-bound intermediates involved in fungal polyketides, fatty acids, and other non-ribosomal peptide formation, which are highly implicated in the construction of aflatoxin biosynthesis. The promising results prompt us to use mango seed extract as a cheap source of gallotannin components as a safe feed additive for reducing fungal and mycotoxin contamination in animal feeds.</p></div>\",\"PeriodicalId\":17019,\"journal\":{\"name\":\"Journal of Stored Products Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2024-05-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Stored Products Research\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0022474X24001115\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Stored Products Research","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0022474X24001115","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
Pentagalloyl glucose and gallotannins from mangifera Indica L. seed kernel extract as a promising antifungal feed additive, in vitro, and in silico studies
Recently, the valorization of agro-industrial wastes through their exploration of their bioactive compounds has become a necessary trend to maximize their applications in various production sectors, particularly animal feeding. In the study, mango seed kernels were used as agro-industrial wastes to extract gallotannins fraction (GF) and pentagalloyl glucose (PGG). The GF and PGG were mixed with poultry feeds to investigate their potential ability to control Aspergillus flavus (A. flavus) growth and inhibit its production of aflatoxins (AFs). Ketoconazole was used as an antifungal standard. GF and PGG combat the growth of A. flavus, with minimum inhibition concentration (MIC) of 21.7 ± 1.7 and 20 ± 0 mg/mL, respectively. Moreover, GF and PGG showed promising results in preventing the production of AFs (AFB1, AFB2, AFG1, and AFG2) at 5 and 10 mg/100-g feed. Furthermore, to explain the mode of action of PGG as an antifungal agent, a molecular docking study has been performed against Type 1 Polyketide Synthase (PKS), which is found in many toxigenic Aspergillus species and has a highly active Acyl Carrier Protein (ACP) domain that is implicated in thiol ester-bound intermediates involved in fungal polyketides, fatty acids, and other non-ribosomal peptide formation, which are highly implicated in the construction of aflatoxin biosynthesis. The promising results prompt us to use mango seed extract as a cheap source of gallotannin components as a safe feed additive for reducing fungal and mycotoxin contamination in animal feeds.
期刊介绍:
The Journal of Stored Products Research provides an international medium for the publication of both reviews and original results from laboratory and field studies on the preservation and safety of stored products, notably food stocks, covering storage-related problems from the producer through the supply chain to the consumer. Stored products are characterised by having relatively low moisture content and include raw and semi-processed foods, animal feedstuffs, and a range of other durable items, including materials such as clothing or museum artefacts.