SPEED-E:用于考古遗存中基于珐琅质的性别估计的 "简易提取和消化(-free)"样本制备方案的改进版

IF 2.6 1区 地球科学 Q1 ANTHROPOLOGY
Timothy P. Cleland , Sara A. McGuire , Jared S. Beatrice , Kimberlee S. Moran , Christine A.M. France
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引用次数: 0

摘要

在考虑历史社会中的人口、资源分配和各种基于性别的文化问题时,准确估计考古人类遗骸中的生物性别至关重要。珐琅质古蛋白质组学的最新发展使得我们可以通过与性染色体相连的珐琅质酶原来估计生物性别。当传统的骨学性别估计因不完整、保存不善或幼年而无法进行时,这种方法就非常有优势。在此,我们在古蛋白质组研究中使用的简易提取和消化样品制备(SPEED)方法和直接阶段尖端清理的基础上,开发了釉质的简易提取和免消化样品制备(SPEED-E)。SPEED-E 方法的提取时间与酸蚀刻方法相似,总体上比基于消化的方法短得多,而且样品用量相对较少。这种新方法有助于快速分析样品价值高、样品材料非常有限的大批量样品。利用 SPEED-E,我们能够估算出美国宾夕法尼亚州费城历史上第一浸信会教堂考古人类骨骼遗骸中 89 颗落叶牙和恒牙中 85 颗的性别。不确定的牙齿检测到的淀粉样蛋白有限或没有检测到淀粉样蛋白,这是因为较大的蛋白质可能来自取样的牙本质。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
SPEED-E: A modified version of the sample preparation by Easy extraction and Digestion(-free) protocol for enamel-based sex estimation in archaeological remains

Accurate estimation of biological sex in archaeological human remains is critical when considering demographic, resource partitioning, and various sex-based cultural issues in historic societies. Recent developments in paleoproteomics of enamel have allowed for the estimation of biological sex through sex chromosome-linked amelogenins. This method is highly advantageous when traditional osteological sex estimation is precluded by incompleteness, poor preservation, or juvenile age. Here, we have developed Sample Preparation by Easy Extraction and Digestion-free for Enamel (SPEED-E), building on the Sample Preparation by Easy Extraction and Digestion (SPEED) method and direct stage tip clean-up used in paleoproteomic studies. The SPEED-E protocol is similar in extraction time to acid etching protocols, is overall much shorter than digestion-based protocols, and uses relatively less sample. This new method facilitates a rapid analysis of large sample batches where sample value is high and sample material is very limited. Using SPEED-E, we were able to estimate the sex of 85 of 89 deciduous and permanent teeth from the assemblage of archaeological human skeletal remains from the historic First Baptist Church of Philadelphia, Pennsylvania, USA. The indeterminate teeth had limited or no detected amelogenins because of overprinting larger proteins, likely from sampled dentin.

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来源期刊
Journal of Archaeological Science
Journal of Archaeological Science 地学-地球科学综合
CiteScore
6.10
自引率
7.10%
发文量
112
审稿时长
49 days
期刊介绍: The Journal of Archaeological Science is aimed at archaeologists and scientists with particular interests in advancing the development and application of scientific techniques and methodologies to all areas of archaeology. This established monthly journal publishes focus articles, original research papers and major review articles, of wide archaeological significance. The journal provides an international forum for archaeologists and scientists from widely different scientific backgrounds who share a common interest in developing and applying scientific methods to inform major debates through improving the quality and reliability of scientific information derived from archaeological research.
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