{"title":"南非艾滋病毒呈阳性妇女口腔白色念珠菌菌株多样性和维持情况","authors":"FJ Owotade , Z. Gulube , M. Patel","doi":"10.1016/j.archoralbio.2024.106007","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>This study investigated <em>C. albicans</em> strain diversity and maintenance in the oral cavity of HIV positive women over a 6 month period.</p></div><div><h3>Study design</h3><p><em>C. albicans</em> strains were isolated from 17 HIV positive women at Charlotte Maxeke Academic Hospital, Johannesburg at 3 intervals over a 6 month period. Strains were genotyped using ABC and Multilocus Sequence Typing (MLST) techniques. In the MLST technique, for each strain, a Diploid Sequence Type (DST) number was obtained. Using cluster analysis, an Unweighted Pair Group Method with Arithmetic Mean (UPGMA) dendrogram and a matrix of strain similarities were generated. Strains were also compared to the previous South African isolates documented in the MLST database.</p></div><div><h3>Results</h3><p>Ninety four percent of women carried the same ABC genotype for 6 months. MLST technique, showed that ten women (58.8%) carried the same DST at 2 visits, while seven (41.2%) carried different DST at all visits. Further analysis showed that 64.7% of women were recolonised with different strains and 35.3% carried the same strains of <em>C. albicans</em> with heterozygosity. A total of 40 diploid sequence types were identified of which 27 DSTs were unique to this study group that were added to the MLST database. Most of the strains were closely related to previously isolated strains from South Africa.</p></div><div><h3>Conclusion</h3><p>Recolonization of the oral cavity with different strains and microevolution of the original strains of <em>C. albicans</em> can occur, which can be a potential problem for HIV patients, in whom highly virulent and drug resistant strains can emerge.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"164 ","pages":"Article 106007"},"PeriodicalIF":2.2000,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0003996924001286/pdfft?md5=76a78bbc42293d054758bb37ab985934&pid=1-s2.0-S0003996924001286-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Oral Candida albicans strain diversity and maintenance in HIV positive women in South Africa\",\"authors\":\"FJ Owotade , Z. Gulube , M. Patel\",\"doi\":\"10.1016/j.archoralbio.2024.106007\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p>This study investigated <em>C. albicans</em> strain diversity and maintenance in the oral cavity of HIV positive women over a 6 month period.</p></div><div><h3>Study design</h3><p><em>C. albicans</em> strains were isolated from 17 HIV positive women at Charlotte Maxeke Academic Hospital, Johannesburg at 3 intervals over a 6 month period. Strains were genotyped using ABC and Multilocus Sequence Typing (MLST) techniques. In the MLST technique, for each strain, a Diploid Sequence Type (DST) number was obtained. Using cluster analysis, an Unweighted Pair Group Method with Arithmetic Mean (UPGMA) dendrogram and a matrix of strain similarities were generated. Strains were also compared to the previous South African isolates documented in the MLST database.</p></div><div><h3>Results</h3><p>Ninety four percent of women carried the same ABC genotype for 6 months. MLST technique, showed that ten women (58.8%) carried the same DST at 2 visits, while seven (41.2%) carried different DST at all visits. Further analysis showed that 64.7% of women were recolonised with different strains and 35.3% carried the same strains of <em>C. albicans</em> with heterozygosity. A total of 40 diploid sequence types were identified of which 27 DSTs were unique to this study group that were added to the MLST database. Most of the strains were closely related to previously isolated strains from South Africa.</p></div><div><h3>Conclusion</h3><p>Recolonization of the oral cavity with different strains and microevolution of the original strains of <em>C. albicans</em> can occur, which can be a potential problem for HIV patients, in whom highly virulent and drug resistant strains can emerge.</p></div>\",\"PeriodicalId\":8288,\"journal\":{\"name\":\"Archives of oral biology\",\"volume\":\"164 \",\"pages\":\"Article 106007\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-05-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0003996924001286/pdfft?md5=76a78bbc42293d054758bb37ab985934&pid=1-s2.0-S0003996924001286-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Archives of oral biology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0003996924001286\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of oral biology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0003996924001286","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Oral Candida albicans strain diversity and maintenance in HIV positive women in South Africa
Objective
This study investigated C. albicans strain diversity and maintenance in the oral cavity of HIV positive women over a 6 month period.
Study design
C. albicans strains were isolated from 17 HIV positive women at Charlotte Maxeke Academic Hospital, Johannesburg at 3 intervals over a 6 month period. Strains were genotyped using ABC and Multilocus Sequence Typing (MLST) techniques. In the MLST technique, for each strain, a Diploid Sequence Type (DST) number was obtained. Using cluster analysis, an Unweighted Pair Group Method with Arithmetic Mean (UPGMA) dendrogram and a matrix of strain similarities were generated. Strains were also compared to the previous South African isolates documented in the MLST database.
Results
Ninety four percent of women carried the same ABC genotype for 6 months. MLST technique, showed that ten women (58.8%) carried the same DST at 2 visits, while seven (41.2%) carried different DST at all visits. Further analysis showed that 64.7% of women were recolonised with different strains and 35.3% carried the same strains of C. albicans with heterozygosity. A total of 40 diploid sequence types were identified of which 27 DSTs were unique to this study group that were added to the MLST database. Most of the strains were closely related to previously isolated strains from South Africa.
Conclusion
Recolonization of the oral cavity with different strains and microevolution of the original strains of C. albicans can occur, which can be a potential problem for HIV patients, in whom highly virulent and drug resistant strains can emerge.
期刊介绍:
Archives of Oral Biology is an international journal which aims to publish papers of the highest scientific quality in the oral and craniofacial sciences. The journal is particularly interested in research which advances knowledge in the mechanisms of craniofacial development and disease, including:
Cell and molecular biology
Molecular genetics
Immunology
Pathogenesis
Cellular microbiology
Embryology
Syndromology
Forensic dentistry