Pedro Nacib Jorge-Neto , Fabio de Moraes Francisco , Mario Davi Dias Carneiro , Sergio Ricardo Brito Santos , Letícia Alecho Requena , Sofia Dressel Ramos , Matheus Felix de Goés , Rafael Franco Valle , Fabiana Lucia André Padilha , Helen Colbachini , Rafael Caprioli Gutierrez , Larissa Schneider Brandão Souza , Verônica Takatsuka Manoel , Laura Chrispim Reisfeld , Thyara Deco-Souza , Roberta Ferreira Leite , Cristiane Schilbach Pizzutto
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引用次数: 0
摘要
1.有三分之一的鞘鳃类动物濒临灭绝，对其精子参数和质量进行精确评估是动物保护生殖技术发展的一个阶段。2.计算机辅助精子分析（CASA）是精子分析的黄金标准；然而，目前还没有一种针对鞘鳃类动物的设置。本研究介绍了使用 CASA 系统对鳍鳃亚纲动物精液进行分析的具体方法。4 此外，本研究还提供了鳍鳃亚纲动物精子质量评估的黄金标准技术规程。5 所描述的方法对 14 种不同的淡水和海水鲨鱼、黄貂鱼和鳐鱼的准确率超过 90%，只有在出现两个或两个以上捆绑精子时才会失败。
Specific setup and methodology for computer-assisted sperm analysis (CASA) in evaluating elasmobranch sperm
1.
One-third of elasmobranch species are threatened with extinction, and the precise assessment of their sperm parameters and quality is one stage in the development of reproductive technology for animal conservation.
2.
Computer-Assisted Sperm Analysis (CASA) is the gold standard for sperm analysis; however, there were not one setup established for elasmobranchs.
3.
This study presents the description of a specific setup developed for elasmobranch semen analysis using the CASA system.
4.
In addition, it provides a gold-standard technique protocol for elasmobranch sperm quality assessment.
5.
The described setup presented an accuracy of more than 90 percent in fourteen distinct species of freshwater and marine sharks, stingrays, and rays, failing only when there were two or more bundled sperm.
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