Yao Yu, Lianjing Zhao, Nuo Xu, Xiaolei Liu, Lin Li, Ning Xu, Xue Bai
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A smartphone-based enhanced colorimetric immunoassay for the detection of Trichinella spiralis infection.
Trichinellosis is a serious foodborne and zoonotic parasitic disease caused by Trichinella family. At present, the main on-site detection method for Trichinella spiralis (T. spiralis) infection is the lateral flow assay (LFA). Other diagnostic techniques for this parasite cannot be applied to on-site testing due to their reliance on special instruments. Here, we established an ELISA smartphone-based method for detecting anti-T. spiralis antibodies in pig serum. The use of horseradish peroxidase-labeled goat anti-pig IgG-modified gold nanoparticle (AuNPs@HRP-IgG) effectively increased the sensitivity of the method. The entire reaction was carried out at room temperature without the need for special instruments. A low-cost and portable device for imaging and processing experimental data was also developed. Validation analysis revealed that the specificity of the test was 98.89 %, while its sensitivity was 100.00 %. T. spiralis antibodies could be detected in pig serum beginning at 25 dpi after infection with the muscle larvae. This visual immunosensor facilitates on-site detection of T. spiralis, especially in regions lacking specialized laboratory equipment.
期刊介绍：
The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review.
This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership.
Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.
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