螺旋毛癣菌幼虫排泄物分泌抗原负载 Ca-BTC 金属有机框架对诱发小鼠结肠炎的预防和治疗作用。

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
E M Fawzy, M A Selim, N E Mostafa, R M Abdelhameed, A M Darwish, A M Yousef, M A Alabiad, M N Ibrahim, H M Fawzy, E F Abdel Hamed
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引用次数: 0

摘要

背景:炎症性肠病是一种影响肠道的自身免疫性疾病:炎症性肠病是一种影响肠道的自身免疫性疾病。我们对负载在苯-1,3,5-三羧酸钙金属有机框架(Ca-BTC MOFs)上的螺旋蓟马幼虫(E/S Ags)进行了测试,以确定它们是否可以预防或治疗醋酸诱导的小鼠结肠炎。研究方法将螺旋蓟马幼虫 E/S Ags/Ca-BTC MOFs 分为预防组和治疗组,在小鼠结肠炎发生之前或之后使用。结肠炎发生后第七天,小鼠被宰杀。我们使用多种指标评估了目标抗原对结肠炎进展的影响,包括存活率、疾病活动指数、结肠重量/体重、结肠重量/长度)比率以及结肠宏观和微观损伤评级。对血清样本中的炎症细胞因子(干扰素-γ和白细胞介素-4)、氧化应激标志物丙二醛和谷胱甘肽过氧化物酶的水平进行了评估。在结肠和脾组织中检测了 Foxp3 T-reg 的表达。结果螺旋蓟马幼虫 E/S Ags/Ca-BTC MOFs 对缓解小鼠结肠炎的严重炎症最有效。小鼠的存活率、疾病活动指数评分、结肠重量/长度比、结肠重量/体重比、结肠大体和显微损伤评分均有显著改善。促炎细胞因子(干扰素-γ)和氧化应激标志物(丙二醛)的表达量大幅下降,白细胞介素-4和谷胱甘肽过氧化物酶的表达量显著增加。结肠和脾组织中 Foxp3+ Treg 细胞的表达升高。结论螺旋蓟马幼虫 E/S Ags/Ca-BTC MOFs 对小鼠结肠炎具有最高的抗炎、抗氧化和细胞保护能力,可用于开发新的预防和治疗策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The prophylactic and therapeutic impact of Trichinella spiralis larvae excretory secretory antigens- loaded Ca-BTC metal organic frameworks on induced murine colitis.

Background: Inflammatory bowel disease is an autoimmune disease that affects the gut. T. spiralis larvae (E/S Ags) loaded on calcium-benzene-1,3,5-tricarboxylate metal-organic frameworks (Ca-BTC MOFs) were tested to determine whether they might prevent or cure acetic acid-induced murine colitis. Methods: T. spiralis larvae E/S Ags/Ca-BTC MOFs were used in prophylactic and therapeutic groups to either precede or follow the development of murine colitis. On the seventh day after colitis, mice were slaughtered. The effect of our target antigens on the progress of the colitis was evaluated using a variety of measures, including survival rate, disease activity index, colon weight/bodyweight, colon weight/length) ratios, and ratings for macroscopic and microscopic colon damage. The levels of inflammatory cytokines (interferon-γ and interleukin-4), oxidative stress marker malondialdehyde, and glutathione peroxidase in serum samples were evaluated. Foxp3 T-reg expression was carried out in colonic and splenic tissues. Results: T. spiralis larvae E/S Ags/Ca-BTC MOFs were the most effective in alleviating severe inflammation in murine colitis. The survival rate, disease activity index score, colon weight/length and colon weight/bodyweight ratios, and gross and microscopic colon damage scores have all considerably improved. A large decrease in proinflammatory cytokine (interferon-γ) and oxidative stress marker (malondialdehyde) expression and a significant increase in interleukin-4 and glutathione peroxidase expression were obtained. The expression of Foxp3+ Treg cells was elevated in colonic and splenic tissues. Conclusion: T. spiralis larvae E/S Ags/Ca-BTC MOFs had the highest anti-inflammatory, antioxidant, and cytoprotective capabilities against murine colitis and might be used to develop new preventative and treatment strategies.

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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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