PPARGC1A 基因表达对水牛乳腺上皮细胞产奶量和细胞代谢的调控作用。

IF 1.7 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Animal Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-05-24 DOI:10.1080/10495398.2024.2344210
Seyed Mahdi Hosseini, Ye Tingzhu, Ran Zaohong, Farman Ullah, Aixin Liang, Guohua Hua, Liguo Yang
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引用次数: 0

摘要

PPARGC1A 基因在调节细胞能量代谢(包括适应性产热、线粒体生物生成、脂肪生成、糖元生成和葡萄糖/脂肪酸代谢)方面发挥着基础性作用。在之前的一项研究中,我们的研究小组调查了地中海水牛中与产奶性状相关的七个 SNPs,本研究在此基础上探讨了 PPARGC1A 基因在水牛乳腺上皮细胞(BuMECs)中的调控影响。我们的研究结果表明,PPARGC1A 基因表达的敲除会显著影响水牛乳腺上皮细胞的生长,包括增殖、细胞周期和凋亡。此外,我们还观察到 PPARGC1A 基因敲除后甘油三酯的分泌下调。此外,与产奶有关的关键基因,包括 STATS、BAD、P53、SREBF1 和 XDH 基因在 RNAi 后上调,而 FABP3 基因则下调。此外,沉默 PPARGC1A 基因导致 BuMECs β-酪蛋白合成显著下调。我们的研究证明了 PPARGC1A 基因在调节水牛乳腺细胞生长、脂质和蛋白质代谢中的重要性。鉴于我们之前的研究,本研究强调了该基因在提高水牛产奶效率和整体奶牛生产率方面的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Regulatory impacts of PPARGC1A gene expression on milk production and cellular metabolism in buffalo mammary epithelial cells.

The PPARGC1A gene plays a fundamental role in regulating cellular energy metabolism, including adaptive thermogenesis, mitochondrial biogenesis, adipogenesis, gluconeogenesis, and glucose/fatty acid metabolism. In a previous study, our group investigated seven SNPs in Mediterranean buffalo associated with milk production traits, and the current study builds on this research by exploring the regulatory influences of the PPARGC1A gene in buffalo mammary epithelial cells (BuMECs). Our findings revealed that knockdown of PPARGC1A gene expression significantly affected the growth of BuMECs, including proliferation, cell cycle, and apoptosis. Additionally, we observed downregulated triglyceride secretion after PPARGC1A knockdown. Furthermore, the critical genes related to milk production, including the STATS, BAD, P53, SREBF1, and XDH genes were upregulated after RNAi, while the FABP3 gene, was downregulated. Moreover, Silencing the PPARGC1A gene led to a significant downregulation of β-casein synthesis in BuMECs. Our study provides evidence of the importance of the PPARGC1A gene in regulating cell growth, lipid, and protein metabolism in the buffalo mammary gland. In light of our previous research, the current study underscores the potential of this gene for improving milk production efficiency and overall dairy productivity in buffalo populations.

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来源期刊
Animal Biotechnology
Animal Biotechnology 工程技术-奶制品与动物科学
CiteScore
2.90
自引率
5.40%
发文量
230
审稿时长
>12 weeks
期刊介绍: Biotechnology can be defined as any technique that uses living organisms (or parts of organisms like cells, genes, proteins) to make or modify products, to improve plants, animals or microorganisms for a specific use. Animal Biotechnology publishes research on the identification and manipulation of genes and their products, stressing applications in domesticated animals. The journal publishes full-length articles and short research communications, as well as comprehensive reviews. The journal also provides a forum for regulatory or scientific issues related to cell and molecular biology applied to animal biotechnology. Submissions on the following topics are particularly welcome: - Applied microbiology, immunogenetics and antibiotic resistance - Genome engineering and animal models - Comparative genomics - Gene editing and CRISPRs - Reproductive biotechnologies - Synthetic biology and design of new genomes
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