人脐带间充质干细胞衍生的外泌体可调控肠道 2 型免疫。

Jiajun Wu, Zhen Yang, Daoyuan Wang, Yihui Xiao, Jia Shao, Kaiqun Ren
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引用次数: 0

摘要

背景:hUCMSC-Exo被认为是一种新型无细胞治疗产品,有望用于治疗各种疾病。2型免疫是一种保护性免疫反应,被归类为2型T辅助细胞(Th2),与螺旋体感染和过敏性疾病有关。hUCMSC-Exo 对肠 2 型免疫反应的影响尚不清楚:方法:给 C57BL/6 小鼠注射 H.poly 以建立肠道 2 型免疫反应,并在感染 H.poly 之前或之后用 hUCMSC-Exo 治疗。分离出肠器官组织并与 IL-4 和 hUCMSC-Exo 共同培养。结果:hUCMSC-Exo能显著延迟感染后第7天H.poly在十二指肠粘膜下层的定植,并促进感染后第14天簇细胞和鹅口疮细胞的增生。HUCMSC-Exo 可增强经 IL-4 处理的肠道器官组织中簇细胞的扩增,并促进溶解性细胞的死亡:我们的研究表明,hUCMSC-Exo 可在早期感染阶段提高耐受性,然后增强肠道 2 型免疫反应,从而在 Th2 启动阶段阻止蠕虫,从而使宿主受益。我们的研究结果表明 hUCMSC-Exo 可能是 2 型免疫反应的积极调节剂,这表明 hUCMSC-Exo 对过敏性疾病具有潜在的治疗作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Human Umbilical Cord Mesenchymal Stem Cell-derived Exosome Regulates Intestinal Type 2 Immunity.

Aims: The aim of this study was to investigate the role of human umbilical cord mesenchymal stem cell-derived exosomes (hUCMSC-Exo) in regulating the intestinal type 2 immune response for either protection or therapy.

Background: hUCMSC-Exo was considered a novel cell-free therapeutic product that shows promise in the treatment of various diseases. Type 2 immunity is a protective immune response classified as T-helper type 2 (Th2) cells and is associated with helminthic infections and allergic diseases. The effect of hUCMSC-Exo on intestinal type 2 immune response is not clear.

Method: C57BL/6 mice were used to establish intestinal type 2 immune response by administering of H.poly and treated with hUCMSC-Exo before or after H.poly infection. Intestinal organoids were isolated and co-cultured with IL-4 and hUCMSC-Exo. Then, we monitored the influence of hUCMSC-Exo on type 2 immune response by checking adult worms, the hyperplasia of tuft and goblet cells.

Result: hUCMSC-Exo significantly delays the colonization of H.poly in subserosal layer of duodenum on day 7 post-infection and promotes the hyperplasia of tuft cells and goblet cells on day 14 post-infection. HUCMSC-Exo enhances the expansion of tuft cells in IL-4 treated intestinal organoids, and promotes lytic cell death.

Conclusion: Our study demonstrates hUCMSC-Exo may benefit the host by increasing the tolerance at an early infection stage and then enhancing the intestinal type 2 immune response to impede the helminth during Th2 priming. Our results show hUCMSC-Exo may be a positive regulator of type 2 immune response, suggesting hUCMSC-Exo has a potential therapeutic effect on allergic diseases.

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