在膀胱出口梗阻大鼠模型中,摄入马勃醇对膀胱功能障碍有保护作用。

IF 1.5 4区 医学 Q3 UROLOGY & NEPHROLOGY
Nozomu Miyazaki, Ryota Katsura, Chiaki Ozaki, Tatsuo Suzutani
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引用次数: 0

摘要

研究目的本研究评估了大豆异黄酮的代谢产物马兜铃醇对膀胱出口梗阻(BOO)大鼠膀胱功能障碍的影响。此外,我们还研究了其作为膀胱梗阻神经保护剂的潜力,并讨论了其在控制膀胱过度活动症(OAB)方面的适用性:方法:18 只雄性 Sprague-Dawley 大鼠在饲养期间被分为三组(每组 6 只)。Sham 组和 C-BOO 组接受不含 equol 的饮食,而 E-BOO 组则补充 equol(0.25 克/千克)。8周大时,大鼠接受BOO手术,饲养4周后进行连续膀胱测定。测量尿氧化应激标记物(8-羟基-2'-脱氧鸟苷和丙二醛),并使用苏木精-伊红、马森三色素和免疫组化染色法(髓鞘神经的神经丝蛋白重链、非髓鞘神经的外周蛋白和丙二醛)分析膀胱组织学:与 C-BOO 组相比,Equol 减少了 BOO 诱导的平滑肌层纤维化,显著延长了排尿间隔时间(C-BOO:193 秒,E-BOO:438 秒)并增加了排尿量(C-BOO:0.54 毫升,E-BOO:1.02 毫升)。Equol 可抑制尿液和膀胱组织中丙二醛水平的增加。虽然 C-BOO 组显示平滑肌层内的外周素单独阳性神经纤维减少,但 Equol 有效地减轻了这种减少:结论:Equol 可减少脂质过氧化和膀胱平滑肌层纤维化,对膀胱神经(周围神经)具有神经保护作用,并可防止大鼠出现与 BOO 相关的膀胱功能障碍。食用马勃醇有望预防与BOO相关的OAB。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Protective effect of equol intake on bladder dysfunction in a rat model of bladder outlet obstruction

Objectives

This study evaluates the impact of equol, a metabolite of soy isoflavone, on bladder dysfunction in rats with bladder outlet obstruction (BOO). In addition, we investigate its potential as a neuroprotective agent for the obstructed bladder and discuss its applicability in managing overactive bladder (OAB).

Methods

Eighteen male Sprague–Dawley rats were divided into three groups (six rats per group) during the rearing period. The Sham and C-BOO groups received an equol-free diet, while the E-BOO group received equol supplementation (0.25 g/kg). At 8 weeks old, rats underwent BOO surgery, followed by continuous cystometry after 4 weeks of rearing. The urinary oxidative stress markers (8-hydroxy-2′-deoxyguanosine and malondialdehyde) were measured, and the bladder histology was analyzed using hematoxylin–eosin, Masson's trichrome, and immunohistochemical staining (neurofilament heavy chain for myelinated nerves, peripherin for unmyelinated nerves, and malondialdehyde).

Results

Equol reduced BOO-induced smooth muscle layer fibrosis, significantly prolonged the micturition interval (C-BOO: 193 s, E-BOO: 438 s) and increased the micturition volume (C-BOO: 0.54 mL, E-BOO: 1.02 mL) compared to the C-BOO group. Equol inhibited the increase in urinary and bladder tissue malondialdehyde levels. While the C-BOO group exhibited reduced peripherin alone positive nerve fibers within the smooth muscle layer, equol effectively attenuated this decline.

Conclusions

Equol reduces lipid peroxidation and smooth muscle layer fibrosis in the bladder and exhibited neuroprotective effects on bladder nerves (peripheral nerves) and prevented the development of bladder dysfunction associated with BOO in rats. Consumption of equol is promising for the prevention of OAB associated with BOO.

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来源期刊
LUTS: Lower Urinary Tract Symptoms
LUTS: Lower Urinary Tract Symptoms UROLOGY & NEPHROLOGY-
CiteScore
3.00
自引率
7.70%
发文量
52
审稿时长
>12 weeks
期刊介绍: LUTS is designed for the timely communication of peer-reviewed studies which provides new clinical and basic science information to physicians and researchers in the field of neurourology, urodynamics and urogynecology. Contributions are reviewed and selected by a group of distinguished referees from around the world, some of whom constitute the journal''s Editorial Board. The journal covers both basic and clinical research on lower urinary tract dysfunctions (LUTD), such as overactive bladder (OAB), detrusor underactivity, benign prostatic hyperplasia (BPH), bladder outlet obstruction (BOO), urinary incontinence, pelvic organ prolapse (POP), painful bladder syndrome (PBS), as well as on other relevant conditions. Case reports are published only if new findings are provided. LUTS is an official journal of the Japanese Continence Society, the Korean Continence Society, and the Taiwanese Continence Society. Submission of papers from all countries are welcome. LUTS has been accepted into Science Citation Index Expanded (SCIE) with a 2011 Impact Factor.
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