姜黄素与鼠李糖乳杆菌 GG 的组合能抑制 SCC-9 人口腔鳞状细胞癌细胞的活力并诱导其凋亡。

IF 3.3 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE
Chatchaphan Udompatanakorn, Panan Ratthawongjirakul
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引用次数: 0

摘要

本研究旨在评估姜黄素与鼠李糖乳杆菌 GG 无细胞上清液(LGG CFS)联合使用对 SCC-9 口腔鳞状细胞癌(OSCC)细胞增殖和诱导凋亡的影响。姜黄素(40 µg/ml)和 25% v/v LGG CFS(108 CFU/ml),无论是单独使用还是联合使用,都能显著降低 SCC-9 细胞和正常人牙龈成纤维细胞(HGF)的活力。有趣的是,低剂量姜黄素(5 µg/ml)和 25% v/v LGG CFS(106 CFU/ml)的组合对 HGF 细胞没有影响,但却能明显抑制 SCC-9 细胞的活力(p p p p
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A Combination of Curcumin and Lactobacillus rhamnosus GG Inhibits Viability and Induces Apoptosis in SCC-9 Human Oral Squamous Cell Carcinoma Cells.

The aim of this study was to evaluate the effect of curcumin combined with Lactobacillus rhamnosus GG cell-free supernatant (LGG CFS) on the proliferation and induction of apoptosis in SCC-9 oral squamous cell carcinoma (OSCC) cells. Curcumin (40 µg/ml) and 25% v/v LGG CFS (108 CFU/ml), both alone and in a combination regimen, significantly decreased the viability of SCC-9 cells and normal human gingival fibroblast (HGF) cells. Interestingly, the combination of low doses of curcumin (5 µg/ml) and 25% v/v LGG CFS (106 CFU/ml) had no effect on the HGF cells but significantly inhibited the viability of SCC-9 cells (p < 0.05). Flow cytometric analysis revealed that SCC-9 cells treated with the combination of low-dose curcumin and low-dose LGG CFS had a higher apoptotic rate than the cells in the control group and the single treatment groups (p < 0.05). The combined treatment also significantly increased the Bax/Bcl2 mRNA and protein expression in SCC-9 cells (p < 0.05) but not in HGF cells, indicating the underlying mechanism of the combination regimen. There was no significant difference in caspase-3 protein expression or the Bcl-xL/Bak and Mcl-1/Bak ratios between the treatment and control groups in both cell lines. These findings suggested that the coadministration of curcumin and LGG could exhibit anticancer effects in SCC-9 cells without causing toxicity to normal fibroblast cells.

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来源期刊
Journal of Evidence-based Integrative Medicine
Journal of Evidence-based Integrative Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
5.90
自引率
0.00%
发文量
43
审稿时长
15 weeks
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