左旋肉碱通过一氧化氮途径对氯喹诱发的瘙痒症具有抗瘙痒作用。

IF 2.8 3区 医学 Q2 PHARMACOLOGY & PHARMACY
Kiran Seemab, Arif-Ullah Khan, Muhammad Imran Khan, Neelum Gul Qazi, Amber Mahmood Minhas, Fawad Ali
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引用次数: 0

摘要

背景:瘙痒是与各种皮肤病和全身性疾病相关的一种令人痛苦的症状。左旋肉碱(β-β-羟基-γ-三甲基氨基丁酸)是一种天然存在的物质,它控制着许多生理过程。本研究旨在确定左旋肉碱通过一氧化氮依赖机制发挥抗瘙痒作用:方法:氯喹诱发的瘙痒症是研究可能的治疗干预措施的实验模型。在这项研究中,我们评估了左旋肉碱在氯喹诱发的瘙痒症模型中对抗氧化应激、一氧化氮和炎症细胞因子的功效:结果:与疾病组相比,左旋肉碱治疗明显减少了搔抓行为(****P ***P ***P 结论:这些发现突出了左旋肉碱的治疗作用:这些发现凸显了左旋肉碱在缓解氯喹诱发的瘙痒症方面的治疗效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anti-pruritic effect of L-carnitine against chloroquine-induced pruritus mediated via nitric oxide pathway.

Background: Pruritus, or itching, is a distressing symptom associated with various dermatological and systemic diseases. L-carnitine (βeta hydroxy-γ-tri methyl amino-butyric acid), is a naturally occurring substance, it controls numerous physiological processes. The present research aims to identify L-carnitine for its anti-pruritic effect via nitric oxide-dependent mechanism.

Methods: Chloroquine-induced pruritus serves as an experimental model to investigate possible therapeutic interventions. In this study, we evaluated the efficacy of L-carnitine in combating oxidative stress, nitric oxide, and inflammatory cytokines in a chloroquine-induced pruritus model.

Results: L-carnitine treatment significantly reduced scratching behavior compared to the disease group (***P < 0.001 vs. chloroquine group), indicating its antipruritic potential. The markers of oxidative stress, GST, GSH, Catalase, and LPO were dysregulated in the disease model, but administration of L-carnitine restored GST, GSH, and Catalase levels and decreased LPO levels (***P < 0.001 vs. chloroquine group), thereby alleviating oxidative stress. L-carnitine also reduced nitric oxide synthase (NOS) activity, suggesting that it modulates nitric oxide signaling pathways involved in pruritus. In addition, L-carnitine lowered levels of pro-inflammatory cytokines such as tumor necrosis factor-alpha (TNF-α), inflammatory marker nuclear factor kappa B (p-NFκB) and also reduces an inflammatory enzyme, cyclooxygenase-2 (COX-2), determined by ELISA (Enzyme-Linked Immunosorbent Assay) (***P < 0.001 vs. chloroquine group). It downregulates nNOS mRNA expression confirmed by real-time polymerase chain reaction (RT-PCR).

Conclusion: These findings highlight the therapeutic effects of L-carnitine in alleviating chloroquine-induced pruritus.

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来源期刊
BMC Pharmacology & Toxicology
BMC Pharmacology & Toxicology PHARMACOLOGY & PHARMACYTOXICOLOGY&nb-TOXICOLOGY
CiteScore
4.80
自引率
0.00%
发文量
87
审稿时长
12 weeks
期刊介绍: BMC Pharmacology and Toxicology is an open access, peer-reviewed journal that considers articles on all aspects of chemically defined therapeutic and toxic agents. The journal welcomes submissions from all fields of experimental and clinical pharmacology including clinical trials and toxicology.
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