Andrea Sanjuan-Badillo, León P Martínez-Castilla, Ricardo García-Sandoval, Patricia Ballester, Cristina Ferrándiz, Maria de la Paz Sanchez, Berenice García-Ponce, Adriana Garay-Arroyo, Elena R Álvarez-Buylla
{"title":"HDAC 与 MADS-结构域蛋白的相互作用:拟南芥中 HDA15 和 XAL1 的案例研究。","authors":"Andrea Sanjuan-Badillo, León P Martínez-Castilla, Ricardo García-Sandoval, Patricia Ballester, Cristina Ferrándiz, Maria de la Paz Sanchez, Berenice García-Ponce, Adriana Garay-Arroyo, Elena R Álvarez-Buylla","doi":"10.1080/15592324.2024.2353536","DOIUrl":null,"url":null,"abstract":"<p><p>Cellular behavior, cell differentiation and ontogenetic development in eukaryotes result from complex interactions between epigenetic and classic molecular genetic mechanisms, with many of these interactions still to be elucidated. Histone deacetylase enzymes (HDACs) promote the interaction of histones with DNA by compacting the nucleosome, thus causing transcriptional repression. MADS-domain transcription factors are highly conserved in eukaryotes and participate in controlling diverse developmental processes in animals and plants, as well as regulating stress responses in plants. In this work, we focused on finding out putative interactions of <i>Arabidopsis thaliana</i> HDACs and MADS-domain proteins using an evolutionary perspective combined with bioinformatics analyses and testing the more promising predicted interactions through classic molecular biology tools. Through bioinformatic analyses, we found similarities between HDACs proteins from different organisms, which allowed us to predict a putative protein-protein interaction between the <i>Arabidopsis thaliana</i> deacetylase HDA15 and the MADS-domain protein XAANTAL1 (XAL1). The results of two-hybrid and Bimolecular Fluorescence Complementation analysis demonstrated <i>in vitro</i> and <i>in vivo</i> HDA15-XAL1 interaction in the nucleus. Likely, this interaction might regulate developmental processes in plants as is the case for this type of interaction in animals.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"19 1","pages":"2353536"},"PeriodicalIF":0.0000,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11110687/pdf/","citationCount":"0","resultStr":"{\"title\":\"HDACs MADS-domain protein interaction: a case study of HDA15 and XAL1 in <i>Arabidopsis thaliana</i>.\",\"authors\":\"Andrea Sanjuan-Badillo, León P Martínez-Castilla, Ricardo García-Sandoval, Patricia Ballester, Cristina Ferrándiz, Maria de la Paz Sanchez, Berenice García-Ponce, Adriana Garay-Arroyo, Elena R Álvarez-Buylla\",\"doi\":\"10.1080/15592324.2024.2353536\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Cellular behavior, cell differentiation and ontogenetic development in eukaryotes result from complex interactions between epigenetic and classic molecular genetic mechanisms, with many of these interactions still to be elucidated. Histone deacetylase enzymes (HDACs) promote the interaction of histones with DNA by compacting the nucleosome, thus causing transcriptional repression. MADS-domain transcription factors are highly conserved in eukaryotes and participate in controlling diverse developmental processes in animals and plants, as well as regulating stress responses in plants. In this work, we focused on finding out putative interactions of <i>Arabidopsis thaliana</i> HDACs and MADS-domain proteins using an evolutionary perspective combined with bioinformatics analyses and testing the more promising predicted interactions through classic molecular biology tools. Through bioinformatic analyses, we found similarities between HDACs proteins from different organisms, which allowed us to predict a putative protein-protein interaction between the <i>Arabidopsis thaliana</i> deacetylase HDA15 and the MADS-domain protein XAANTAL1 (XAL1). The results of two-hybrid and Bimolecular Fluorescence Complementation analysis demonstrated <i>in vitro</i> and <i>in vivo</i> HDA15-XAL1 interaction in the nucleus. Likely, this interaction might regulate developmental processes in plants as is the case for this type of interaction in animals.</p>\",\"PeriodicalId\":94172,\"journal\":{\"name\":\"Plant signaling & behavior\",\"volume\":\"19 1\",\"pages\":\"2353536\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-12-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11110687/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant signaling & behavior\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/15592324.2024.2353536\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/5/21 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant signaling & behavior","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/15592324.2024.2353536","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/5/21 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
HDACs MADS-domain protein interaction: a case study of HDA15 and XAL1 in Arabidopsis thaliana.
Cellular behavior, cell differentiation and ontogenetic development in eukaryotes result from complex interactions between epigenetic and classic molecular genetic mechanisms, with many of these interactions still to be elucidated. Histone deacetylase enzymes (HDACs) promote the interaction of histones with DNA by compacting the nucleosome, thus causing transcriptional repression. MADS-domain transcription factors are highly conserved in eukaryotes and participate in controlling diverse developmental processes in animals and plants, as well as regulating stress responses in plants. In this work, we focused on finding out putative interactions of Arabidopsis thaliana HDACs and MADS-domain proteins using an evolutionary perspective combined with bioinformatics analyses and testing the more promising predicted interactions through classic molecular biology tools. Through bioinformatic analyses, we found similarities between HDACs proteins from different organisms, which allowed us to predict a putative protein-protein interaction between the Arabidopsis thaliana deacetylase HDA15 and the MADS-domain protein XAANTAL1 (XAL1). The results of two-hybrid and Bimolecular Fluorescence Complementation analysis demonstrated in vitro and in vivo HDA15-XAL1 interaction in the nucleus. Likely, this interaction might regulate developmental processes in plants as is the case for this type of interaction in animals.