Min Kuk Suh, Jong- Sik Jin, Hyo Eun Do, Ji-Sun Kim, Mi Kyung Eom, Han Sol Kim, Jung- Sook Lee
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引用次数: 0
摘要
从 BALB/c 小鼠粪便中分离出一种新型严格厌氧细菌 JBNU-10 T 菌株。JBNU-10 T菌株的细胞呈革兰氏染色阳性,无运动性,呈杆状。基于 16S rRNA 基因序列的系统进化分析表明,菌株 JBNU-10 T 属于 Adlercreutzia 属,与 Adlercreutzia muris WCA-131-CoC-2 T 关系密切(95.90%)。菌株 JBNU-10 T 的基因组测序结果显示,其基因组大小为 2,790,983 bp,DNA G + C 含量为 69.4 mol%。它总共包含 2,266 个 CDS、5 个 rRNA 基因和 49 个 tRNA 基因。根据所获得的数据,菌株 JBNU-10 T 与近缘种的 ANI 值低于 77.6- 67.7%,dDDH 值低于 23.8%。菌株 JBNU-10 T 的主要脂肪酸为异 C16:0 DMA、C18:1 CIS 9 FAME 和 C18:0 DMA,并具有 DMMK-6。发酵的主要最终产物是丙酸和乙酸。根据系统发育、生理和化学分类学特征,菌株 JBNU-10 T 代表 Adlercreutzia 属的一个新种。模式菌株为 JBNU-10 T(= KCTC 25028 T = CCUG 75610 T)。
Adlercreutzia faecimuris sp. nov., producing propionate and acetate isolated from mouse feces
A novel strictly anaerobic bacterium, strain JBNU-10 T, was isolated from BALB/c mouse feces. Cells of the strain JBNU-10 T were Gram-stain positive, non-motile and rod-shaped. Optimum growth occurred at 37℃, with 1% (w/v) NaCl and at pH 7. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain JBNU-10 T belonged to the genus Adlercreutzia and were closely related to Adlercreutzia muris WCA-131-CoC-2 T (95.90%). The genome sequencing of strain JBNU-10 T revealed a genome size of 2,790,983 bp, a DNA G + C content of 69.4 mol%. It contains a total of 2,266 CDSs, 5 rRNA genes and 49 tRNA genes. According to the data obtained strain JBNU-10 T shared ANI value below 77.6- 67.7%, dDDH value below 23.8% with the closely type species. Strain JBNU-10 T possessed iso-C16:0 DMA, C18:1 CIS 9 FAME, and C18:0 DMA as the major fatty acids and had DMMK-6. The major end products of fermentation is propionate and acetate. Based on phylogenetic, physiological and chemotaxonomic characteristics, strain JBNU-10 T represent a novel species of the genus Adlercreutzia. The type strain is JBNU-10 T (= KCTC 25028 T = CCUG 75610 T).
期刊介绍:
Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.