Tunturibacter gen. nov. 的基因组分析和描述扩大了苔原土壤中 Terriglobia 的多样性。

IF 4.3 2区 生物学 Q2 MICROBIOLOGY
Adriana Messyasz, Minna K. Männistö, Lee J. Kerkhof, Max M. Häggblom
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引用次数: 0

摘要

北极苔原生态系统温度升高,导致土壤有机物质的微生物呼吸速率加快,从而释放出二氧化碳和甲烷。要了解这种微生物活动的影响,就必须更好地描述北极土壤中微生物群落的多样性。我们的目标是完善我们对 Terriglobia 系统发育多样性的理解,这是酸性杆菌门中一个常见但难以捉摸的群体。这将有助于我们将这种多样性与碳和氮利用模式的变化联系起来。我们使用长读数牛津纳米孔 MinION 序列与元基因组短读数序列相结合来组装完整的酸性杆菌群基因组。这使我们能够建立多焦点系统进化和注释泛基因组标记,以区分来自几种苔原土壤分离物的酸性杆菌菌株。我们发现了一个系统发育群,其中包含以前与地衣埃德拉弗氏菌(Edaphobacter lichenicola)相关的四个新物种。我们得出结论,该聚类代表了一个新属,并将其命名为 Tunturibacter。我们描述了四个新物种:Tunturibacter lichenicola comb.nov.、Tunturibacter empetritectus sp.nov.、Tunturibacter gelidoferens sp.nov.和 Tunturibacter psychrotolerans sp.nov.。我们希望通过发现Terriglobia中的新物种和新菌株,并提高其系统发育定位的准确性,来加深我们对这一复杂门类的理解,并揭示极地土壤中微生物群落的形成机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Genome analysis and description of Tunturibacter gen. nov. expands the diversity of Terriglobia in tundra soils

Genome analysis and description of Tunturibacter gen. nov. expands the diversity of Terriglobia in tundra soils

Increased temperatures in Arctic tundra ecosystems are leading to higher microbial respiration rates of soil organic matter, resulting in the release of carbon dioxide and methane. To understand the effects of this microbial activity, it is important to better characterize the diverse microbial communities in Arctic soil. Our goal is to refine our understanding of the phylogenetic diversity of Terriglobia, a common but elusive group within the Acidobacteriota phylum. This will help us link this diversity to variations in carbon and nitrogen usage patterns. We used long-read Oxford Nanopore MinION sequences in combination with metagenomic short-read sequences to assemble complete Acidobacteriota genomes. This allowed us to build multi-locus phylogenies and annotate pangenome markers to distinguish Acidobacteriota strains from several tundra soil isolates. We identified a phylogenetic cluster containing four new species previously associated with Edaphobacter lichenicola. We conclude that this cluster represents a new genus, which we have named Tunturibacter. We describe four new species: Tunturibacter lichenicola comb. nov., Tunturibacter empetritectus sp. nov., Tunturibacter gelidoferens sp. nov., and Tunturibacter psychrotolerans sp. nov. By uncovering new species and strains within the Terriglobia and improving the accuracy of their phylogenetic placements, we hope to enhance our understanding of this complex phylum and shed light on the mechanisms that shape microbial communities in polar soils.

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来源期刊
Environmental microbiology
Environmental microbiology 环境科学-微生物学
CiteScore
9.90
自引率
3.90%
发文量
427
审稿时长
2.3 months
期刊介绍: Environmental Microbiology provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens
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