临床分离的耐抗生素金黄色葡萄球菌 (MRSA)、大肠杆菌 (ESBL) 和 1379 号不动杆菌菌株的表型和分子特征。

IF 2.1 4区 生物学 Q3 MICROBIOLOGY
Brazilian Journal of Microbiology Pub Date : 2024-09-01 Epub Date: 2024-05-22 DOI:10.1007/s42770-024-01347-5
Daraksha Iram, Manish Singh Sansi, Anil Kumar Puniya, Kamal Gandhi, Sunita Meena, Shilpa Vij
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引用次数: 0

摘要

引起医院内感染的抗生素耐药细菌是全球健康的重大隐患。本研究重点研究了金黄色葡萄球菌(MRSA 1418)、大肠杆菌(ESBL 1384)和醋酸杆菌(Acinetobacter 1379)的非耐药菌株和临床耐药菌株的脂质特征。主要目的是研究脂质特征、疏水性和抗生素耐药性之间的关系,从而确定从败血症和尿路感染(UTI)患者中分离出的菌株的致病潜力和耐药性因素。研究包括各种测试,如遵循 CLSI 指南的抗菌药敏感性测试、生化测试、生物膜测试和疏水性测试。此外,还采用气相色谱质谱法(GC-MS)和气相色谱-火焰离子化检测器(GC-FID)分析法进行脂质分析和成分分析。临床分离出的耐药菌株(MRSA-1418、ESBL-1384 和 1379)的耐药表型分别为 81.80%、27.6% 和 63.6%,多重抗生素耐药指数分别为 0.81、0.27 和 0.63。值得注意的是,与非耐药菌株相比,表现出耐药性的 MRSA-1418 菌株的溶血素、细胞表面疏水性、生物膜指数和自我聚集表型水平明显更高。使用实时定量 PCR(qPCR)进行基因表达分析。表明与非耐药菌株相比,MRSA-1418(pgaA、pgaC 和 pgaB)和 1379 的细胞间粘附生物膜相关基因(icaA、icaC 和 icaD)在 24 小时后的表达水平升高。扫描电子显微镜(SEM)用于结构研究。这些发现为了解生物膜在抗生素耐药性中的作用提供了宝贵的见解,并提出了对付抗生素耐药细菌的潜在目标途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Phenotypic and molecular characterization of clinically isolated antibiotics-resistant S. aureus (MRSA), E. coli (ESBL) and Acinetobacter 1379 bacterial strains.

Phenotypic and molecular characterization of clinically isolated antibiotics-resistant S. aureus (MRSA), E. coli (ESBL) and Acinetobacter 1379 bacterial strains.

Antibiotic-resistant bacteria causing nosocomial infections pose a significant global health concern. This study focused on examining the lipid profiles of both non-resistant and clinically resistant strains of Staphylococcus aureus (MRSA 1418), E. coli (ESBL 1384), and Acinetobacter 1379. The main aim was to investigate the relationship between lipid profiles, hydrophobicity, and antibiotic resistance so as to identify the pathogenic potential and resistance factors of strains isolated from patients with sepsis and urinary tract infections (UTIs). The research included various tests, such as antimicrobial susceptibility assays following CLSI guidelines, biochemical tests, biofilm assays, and hydrophobicity assays. Additionally, gas chromatography mass spectrometry (GC-MS) and GC-Flame Ionization Detector (GC-FID) analysis were used for lipid profiling and composition. The clinically isolated resistant strains (MRSA-1418, ESBL-1384, and Acinetobacter 1379) demonstrated resistance phenotypes of 81.80%, 27.6%, and 63.6%, respectively, with a multiple antibiotic resistance index of 0.81, 0.27, and 0.63. Notably, the MRSA-1418 strain, which exhibited resistance, showed significantly higher levels of hemolysin, cell surface hydrophobicity, biofilm index, and a self-aggregative phenotype compared to the non-resistant strains. Gene expression analysis using quantitative real-time PCR (qPCR). Indicated elevated expression levels of intercellular adhesion biofilm-related genes (icaA, icaC, and icaD) in MRSA-1418 (pgaA, pgaC, and pgaB) and Acinetobacter 1379 after 24 h compared to non-resistant strains. Scanning electron microscopy (SEM) was employed for structural investigation. These findings provide valuable insights into the role of biofilms in antibiotic resistance and suggest potential target pathways for combating antibiotic-resistant bacteria.

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来源期刊
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology 生物-微生物学
CiteScore
4.10
自引率
4.50%
发文量
216
审稿时长
1.0 months
期刊介绍: The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
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