一种可能与圈养北美雪豹(Panthera uncia)皮肤斑块和鳞状细胞癌相关的新型乳头状瘤病毒。

IF 2.3 2区 农林科学 Q2 PATHOLOGY
Veterinary Pathology Pub Date : 2024-11-01 Epub Date: 2024-05-21 DOI:10.1177/03009858241254340
Mandy Womble, Shaina Weingart, Susan May, Michael Garner, Jennifer Luff
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引用次数: 0

摘要

皮肤斑块和鳞状细胞癌(SCC)在圈养的北美雪豹(Panthera uncia)中很常见。我们的目的是确定这些病变是否可能与乳头状瘤病毒有关。我们使用乳头状瘤病毒的退化引物对 3 块皮肤斑块进行了聚合酶链式反应(PCR)。结果发现了一种与猫乳头瘤病毒 2 有 76% 相同序列的新型乳头瘤病毒。对 5 个皮肤 SCC 样本和 7 个正常皮肤样本进行了特异性 PCR 检测,结果均为阳性。对这种假定的新型病毒进行了原位杂交,结果在皮肤斑块的增生细胞内(n = 3)和皮肤 SCC 样本的肿瘤细胞内(n = 5)发现了强烈的杂交信号。正常皮肤内未发现杂交信号。最终,确定SLs斑块和SCC发病的病毒病原体将有助于指导治疗干预,并为开发预防性疫苗奠定基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A putatively novel papillomavirus associated with cutaneous plaques and squamous cell carcinoma in captive North American snow leopards (Panthera uncia).

Cutaneous plaques and squamous cell carcinoma (SCC) are common in captive North American snow leopards (SLs) (Panthera uncia). Our objective was to determine whether these lesions are potentially associated with papillomavirus(es). Polymerase chain reaction (PCR) was performed on 3 cutaneous plaques using degenerate primers for papillomaviruses. A putatively novel papillomavirus was identified that shared 76% sequence identity to Felis catus papillomavirus 2. Specific PCR for this virus was performed on 5 cutaneous SCC samples and 7 normal skin samples, which were all positive. In situ hybridization for this putatively novel virus was performed, which revealed strong hybridization signals within hyperplastic cells in cutaneous plaques (n = 3) and within neoplastic cells in cutaneous SCC samples (n = 5). No hybridization signals were identified within normal skin. Ultimately, identification of a causal viral agent in the development of plaques and SCC in SLs will help guide therapeutic intervention and lay the foundation for development of prophylactic vaccines.

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来源期刊
Veterinary Pathology
Veterinary Pathology 农林科学-病理学
CiteScore
4.70
自引率
8.30%
发文量
99
审稿时长
2 months
期刊介绍: Veterinary Pathology (VET) is the premier international publication of basic and applied research involving domestic, laboratory, wildlife, marine and zoo animals, and poultry. Bridging the divide between natural and experimental diseases, the journal details the diagnostic investigations of diseases of animals; reports experimental studies on mechanisms of specific processes; provides unique insights into animal models of human disease; and presents studies on environmental and pharmaceutical hazards.
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