使用 DIA 方法对患有和未患有幼儿龋齿的儿童进行唾液蛋白质组学比较分析：试点研究。

IF 2.1 4区生物学 Q3 BIOCHEMICAL RESEARCH METHODS

PROTEOMICS – Clinical Applications Pub Date : 2024-11-01 Epub Date: 2024-05-20 DOI:10.1002/prca.202400006

Jinxiang Ye, Fangfang Zhang, Zhouyuan Luo, Xiaoyan Ou

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引用次数: 0

摘要

目的筛选不同严重程度的儿童早期龋齿（ECC）患者唾液中的差异表达蛋白（DEPs）：方法：采用数据独立采集技术（DIA）对不同严重程度的幼儿龋患儿唾液中的蛋白质组进行分析。本研究共纳入了 12 名 3-5 岁的学龄前儿童：DEPs筛选结果显示，H组与LC组之间共发现34个DEPs，包括18个上调蛋白和16个下调蛋白；H组与HC组之间共筛选出34个DEPs，包括17个上调蛋白和17个下调蛋白；LC组与HC组之间共筛选出42个DEPs，包括18个上调蛋白和24个下调蛋白。在这些DEPs中，我们筛选出了几个可能在ECC中发挥作用的关键蛋白，如MK、组蛋白H4、TGFβ3、ZG16B、MUC20和SMR-3B：结论：唾液蛋白是龋病的重要宿主因子，在ECC儿童和健康儿童的唾液中存在表达差异。特定的DEPs有望成为诊断ECC的潜在生物标志物。

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Comparative salivary proteomics analysis of children with and without early childhood caries using the DIA approach: A pilot study.

Objective: To screen differentially expressed proteins (DEPs) in the saliva of Early childhood caries (ECC) with different degrees of severity.

Methods: The proteomic profiles of salivary of children with ECC of varying severity by data independent acquisition data independent acquisition (DIA) technique. A total of 12 preschool children aged 3-5 years were included in this study.

Results: In this study, a total of 15,083 peptides and 1944 proteins were quantified; The results of DEPs screening showed that 34 DEPs were identified between the group H and the group LC, including 18 up-regulated proteins and 16 down-regulated proteins; 34 DEPs were screened between the group H and the group HC, including 17 up-regulated proteins and 17 down-regulated proteins; 42 DEPs were screened between the group LC and the group HC, including 18 up-regulated proteins and 24 down-regulated proteins. Among these DEPs, we screened several key proteins that may play a role in ECC, such as MK, histone H4, TGFβ3, ZG16B, MUC20, and SMR-3B.

Conclusion: Salivary proteins, as important host factors of caries, are differentially expressed between the saliva of ECC children and healthy children. Specific DEPs are expected to become potential biomarkers for the diagnosis of ECC.

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来源期刊

PROTEOMICS – Clinical Applications 医学-生化研究方法

CiteScore

5.20

自引率

5.00%

发文量

审稿时长

1 months

期刊介绍： PROTEOMICS - Clinical Applications has developed into a key source of information in the field of applying proteomics to the study of human disease and translation to the clinic. With 12 issues per year, the journal will publish papers in all relevant areas including: -basic proteomic research designed to further understand the molecular mechanisms underlying dysfunction in human disease -the results of proteomic studies dedicated to the discovery and validation of diagnostic and prognostic disease biomarkers -the use of proteomics for the discovery of novel drug targets -the application of proteomics in the drug development pipeline -the use of proteomics as a component of clinical trials.