大肠杆菌单链 DNA 结合蛋白原型的分子研究。

IF 6.2 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Critical Reviews in Biochemistry and Molecular Biology Pub Date : 2024-02-01 Epub Date: 2024-05-21 DOI:10.1080/10409238.2024.2330372

Nina J Bonde, Alexander G Kozlov, Michael M Cox, Timothy M Lohman, James L Keck

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引用次数: 0

摘要

大肠杆菌的 SSB 蛋白可在 DNA 代谢过程中的任何地方与单链 DNA 结合。根据条件的不同，SSB 有几种不同的结合模式。在发挥作用的过程中，SSB 会在 ssDNA 上扩散，并在 ssDNA 的不同区段之间快速转移。SSB 与许多其他参与 DNA 代谢的蛋白质相互作用，迄今已定义了 22 种这样的 SSB 相互作用蛋白质或 SIP。这些相互作用主要涉及 SSB 的无序和保守的 C 端残基。当 SSB 未与 ssDNA 结合时，可聚集形成相分离的生物分子凝聚物。本综述概述了目前对 SSB 特性及其多种分子间相互作用功能意义的理解。

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Molecular insights into the prototypical single-stranded DNA-binding protein from E. coli.

The SSB protein of Escherichia coli functions to bind single-stranded DNA wherever it occurs during DNA metabolism. Depending upon conditions, SSB occurs in several different binding modes. In the course of its function, SSB diffuses on ssDNA and transfers rapidly between different segments of ssDNA. SSB interacts with many other proteins involved in DNA metabolism, with 22 such SSB-interacting proteins, or SIPs, defined to date. These interactions chiefly involve the disordered and conserved C-terminal residues of SSB. When not bound to ssDNA, SSB can aggregate to form a phase-separated biomolecular condensate. Current understanding of the properties of SSB and the functional significance of its many intermolecular interactions are summarized in this review.

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来源期刊

Critical Reviews in Biochemistry and Molecular Biology 生物-生化与分子生物学

CiteScore

14.90

自引率

0.00%

发文量

期刊介绍： As the discipline of biochemistry and molecular biology have greatly advanced in the last quarter century, significant contributions have been made towards the advancement of general medicine, genetics, immunology, developmental biology, and biophysics. Investigators in a wide range of disciplines increasingly require an appreciation of the significance of current biochemical and molecular biology advances while, members of the biochemical and molecular biology community itself seek concise information on advances in areas remote from their own specialties. Critical Reviews in Biochemistry and Molecular Biology believes that well-written review articles prove an effective device for the integration and meaningful comprehension of vast, often contradictory, literature. Review articles also provide an opportunity for creative scholarship by synthesizing known facts, fruitful hypotheses, and new concepts. Accordingly, Critical Reviews in Biochemistry and Molecular Biology publishes high-quality reviews that organize, evaluate, and present the current status of high-impact, current issues in the area of biochemistry and molecular biology. Topics are selected on the advice of an advisory board of outstanding scientists, who also suggest authors of special competence. The topics chosen are sufficiently broad to interest a wide audience of readers, yet focused enough to be within the competence of a single author. Authors are chosen based on their activity in the field and their proven ability to produce a well-written publication.