氧化还原活性灰葫芦提取物通过调节水稻的初级代谢物减轻盐胁迫毒性

IF 5.4 Q1 PLANT SCIENCES
J. Tripathi , M. Pandey , R. Ambolikar , PS Variyar , P. Suprasanna , AK Srivastava
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引用次数: 0

摘要

盐分胁迫被认为是降低植物生长和作物产量的主要有害胁迫之一。因此,人们正在共同努力开发可持续的解决方案,以减少盐分对作物生产力造成的负面影响。有鉴于此,本研究评估了灰葫芦提取物(AGE;0.9 µg/mL)改善水稻(全球主要主粮作物之一)NaCl(100 mM)胁迫的潜力。差异表型分析表明,与对照组相比,在 NaCl 处理下,水稻的存活率和全苗生物量分别下降了 0.27 倍和 0.36 倍,这表明水稻的生长受到了抑制。相比之下,在 NaCl 处理之前用 AGE 预处理 24 小时(AGE24h+NaCl),这些生长属性分别比 NaCl 处理时提高了 1.29 倍和 1.70 倍。AGE 的不同表型与其清除活性氧的固有能力有关,其清除活性氧的能力相当于抗坏血酸的 0.08 倍。在 AGE24h 处理下,超氧自由基积累增加,OsTSPO、OsCBS、OsHKT1;5 和 OsNHX1 等胁迫标记基因表达上调,这也表明 AGE 介导了引诱效应。在 AGE24h+NaCl 处理条件下,这些应激标记基因的表达水平要么保持不变,要么进一步上调。此外,在 AGE24h+NaCl 处理下,抗氧化机制的协调激活和 Na-积累的减少进一步支持了应激的改善。基于 GC-MS 的代谢组学研究表明,脂肪酸、苹果酸、肌醇、阿洛糖、三卤糖和 L- 氧代脯氨酸是 AGE 介导的 NaCl 胁迫改善的关键代谢物。叶面喷施 AGE 与 NaCl 相比,种子产量和千粒重分别增加了 1.13 倍和 1.06 倍,验证了其在农艺学上的可行性。因此,研究结果强调了 AGE 作为一种 "绿色 "生物调节剂在改善水稻 NaCl 胁迫条件方面的应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Redox-active ash gourd extract mitigates salt-stress toxicity through modulation of primary metabolites in rice

Salinity stress is considered as one of the major detrimental stresses for reducing plant growth and crop productivity. Hence, concerted efforts are going on to develop sustainable solutions for reducing salinity-induced negative effects on crop productivity. Given this, the present study evaluated the potential of ash gourd extract (AGE; 0.9 µg/mL) for ameliorating NaCl (100 mM) stress in rice, which is one of the major staple food crops worldwide. The differential phenotyping revealed growth reduction under NaCl treatment, as indicated by 0.27- and 0.36-fold decrease in survival and whole-seedling biomass, respectively, compared with those of control. In contrast, 24 h pre-treatment with AGE before NaCl exposure (AGE24h+NaCl) improved these growth attributes by 1.29- and 1.70-fold, respectively, compared with those of NaCl treatment. The differential phenotype of AGE was associated with its inherent ability to scavenge reactive oxygen species, which was equivalent to 0.08-fold of ascorbic acid. The higher accumulation of superoxide radicals and upregulated expression of stress marker genes including OsTSPO, OsCBS, OsHKT1;5, and OsNHX1 under AGE24h treatment also suggested AGE mediated priming effect. Under AGE24h+NaCl, the expression levels of these stress markers were either maintained or their extent of upregulation was further enhanced. In addition, the coordinated activation of antioxidant machinery and reduced Na-accumulation further supported stress amelioration under AGE24h+NaCl treatment. GC-MS-based metabolomics highlighted fatty acids, malic acid, myo-inositol, allose, trehalose, and L-oxoproline, as key metabolites, associated with AGE-mediated amelioration of NaCl stress. The foliar application of AGE increased seed yield and 1000 seed weight by 1.13- and 1.06-fold, respectively, compared with those of NaCl, validating its agronomic feasibility. Thus, the results highlighted the application of AGE, as a “green” bioregulator for ameliorating NaCl stress conditions in rice.

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来源期刊
Current Plant Biology
Current Plant Biology Agricultural and Biological Sciences-Plant Science
CiteScore
10.90
自引率
1.90%
发文量
32
审稿时长
50 days
期刊介绍: Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.
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