T. M. Janson, L. L. Ramenzoni, C. R. Hatz, U. Schlagenhauf, T. Attin, P. R. Schmidlin
{"title":"Limosilactobacillus reuteri 上清液能减轻人牙龈成纤维细胞对 LPS 的炎症反应，但不能减轻对葡萄糖水平升高的炎症反应。","authors":"T. M. Janson, L. L. Ramenzoni, C. R. Hatz, U. Schlagenhauf, T. Attin, P. R. Schmidlin","doi":"10.1111/jre.13290","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Aim</h3>\n \n <p>We investigated the in vitro effect of <i>Limosilactobacillus reuteri</i> DSM 17938 supernatant on the inflammatory response of human gingival fibroblasts (HGF) challenged by lipopolysaccharide (LPS) or elevated glucose levels.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>HGF were exposed to LPS (1 μg/mL), glucose (5, 12 mM or 25 mM), and dilutions of supernatant prepared from <i>L. reuteri</i> DSM 17938 (0.5 × 10<sup>7</sup>, 1.0 × 10<sup>7</sup>, 2.5 × 10<sup>7</sup>, and 5.0 × 10<sup>7</sup> CFU/mL). After 24 h cell viability and levels of cytokines (<i>IL-1β</i>, <i>IL-6</i> and <i>IL-8</i>) and <i>TLR-2</i> were determined.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>None of the tested <i>L. reuteri</i> (DSM 17938) supernatant concentrations reduced the viability of HGF. Supernatant concentrations (2.5 × 10<sup>7</sup> and 5 × 10<sup>7</sup> CFU/mL) significantly (<i>p</i> < .05) decreased the production of <i>IL-1β</i>, <i>IL-6</i>, <i>IL-8</i>, and <i>TLR-</i>2 in the presence of LPS. In contrast, inflammatory markers were not reduced by <i>L. reuteri</i> supernatant in the presence of glucose. Glucose concentrations of 12 mM and 24 mM still lead to an elevated production of the investigated biochemical mediators.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>While <i>L. reuteri</i> (DSM 17938) supernatant attenuates the inflammatory response of HGF to LPS in a dose-dependent manner, elevated glucose levels suppress this action. These in vitro results support the overall anti-inflammatory efficacy of <i>L. reuteri</i> supplementation in plaque-associated periodontal inflammations.</p>\n </section>\n </div>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":"59 5","pages":"974-981"},"PeriodicalIF":3.4000,"publicationDate":"2024-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jre.13290","citationCount":"0","resultStr":"{\"title\":\"Limosilactobacillus reuteri supernatant attenuates inflammatory responses of human gingival fibroblasts to LPS but not to elevated glucose levels\",\"authors\":\"T. M. Janson, L. L. Ramenzoni, C. R. Hatz, U. Schlagenhauf, T. Attin, P. R. Schmidlin\",\"doi\":\"10.1111/jre.13290\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Aim</h3>\\n \\n <p>We investigated the in vitro effect of <i>Limosilactobacillus reuteri</i> DSM 17938 supernatant on the inflammatory response of human gingival fibroblasts (HGF) challenged by lipopolysaccharide (LPS) or elevated glucose levels.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>HGF were exposed to LPS (1 μg/mL), glucose (5, 12 mM or 25 mM), and dilutions of supernatant prepared from <i>L. reuteri</i> DSM 17938 (0.5 × 10<sup>7</sup>, 1.0 × 10<sup>7</sup>, 2.5 × 10<sup>7</sup>, and 5.0 × 10<sup>7</sup> CFU/mL). After 24 h cell viability and levels of cytokines (<i>IL-1β</i>, <i>IL-6</i> and <i>IL-8</i>) and <i>TLR-2</i> were determined.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>None of the tested <i>L. reuteri</i> (DSM 17938) supernatant concentrations reduced the viability of HGF. Supernatant concentrations (2.5 × 10<sup>7</sup> and 5 × 10<sup>7</sup> CFU/mL) significantly (<i>p</i> < .05) decreased the production of <i>IL-1β</i>, <i>IL-6</i>, <i>IL-8</i>, and <i>TLR-</i>2 in the presence of LPS. In contrast, inflammatory markers were not reduced by <i>L. reuteri</i> supernatant in the presence of glucose. Glucose concentrations of 12 mM and 24 mM still lead to an elevated production of the investigated biochemical mediators.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>While <i>L. reuteri</i> (DSM 17938) supernatant attenuates the inflammatory response of HGF to LPS in a dose-dependent manner, elevated glucose levels suppress this action. These in vitro results support the overall anti-inflammatory efficacy of <i>L. reuteri</i> supplementation in plaque-associated periodontal inflammations.</p>\\n </section>\\n </div>\",\"PeriodicalId\":16715,\"journal\":{\"name\":\"Journal of periodontal research\",\"volume\":\"59 5\",\"pages\":\"974-981\"},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2024-05-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jre.13290\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of periodontal research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jre.13290\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of periodontal research","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jre.13290","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Limosilactobacillus reuteri supernatant attenuates inflammatory responses of human gingival fibroblasts to LPS but not to elevated glucose levels
Aim
We investigated the in vitro effect of Limosilactobacillus reuteri DSM 17938 supernatant on the inflammatory response of human gingival fibroblasts (HGF) challenged by lipopolysaccharide (LPS) or elevated glucose levels.
Methods
HGF were exposed to LPS (1 μg/mL), glucose (5, 12 mM or 25 mM), and dilutions of supernatant prepared from L. reuteri DSM 17938 (0.5 × 107, 1.0 × 107, 2.5 × 107, and 5.0 × 107 CFU/mL). After 24 h cell viability and levels of cytokines (IL-1β, IL-6 and IL-8) and TLR-2 were determined.
Results
None of the tested L. reuteri (DSM 17938) supernatant concentrations reduced the viability of HGF. Supernatant concentrations (2.5 × 107 and 5 × 107 CFU/mL) significantly (p < .05) decreased the production of IL-1β, IL-6, IL-8, and TLR-2 in the presence of LPS. In contrast, inflammatory markers were not reduced by L. reuteri supernatant in the presence of glucose. Glucose concentrations of 12 mM and 24 mM still lead to an elevated production of the investigated biochemical mediators.
Conclusion
While L. reuteri (DSM 17938) supernatant attenuates the inflammatory response of HGF to LPS in a dose-dependent manner, elevated glucose levels suppress this action. These in vitro results support the overall anti-inflammatory efficacy of L. reuteri supplementation in plaque-associated periodontal inflammations.
期刊介绍：
The Journal of Periodontal Research is an international research periodical the purpose of which is to publish original clinical and basic investigations and review articles concerned with every aspect of periodontology and related sciences. Brief communications (1-3 journal pages) are also accepted and a special effort is made to ensure their rapid publication. Reports of scientific meetings in periodontology and related fields are also published.
One volume of six issues is published annually.
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