右美托咪定通过抑制 NF-ĸB 通路缓解大鼠术后急性焦虑症

IF 0.7 4区 医学 Q4 PHARMACOLOGY & PHARMACY
Si-Wei Wei, Lei Wu, Zhen Xiang, Er-You Yu, Zhen Du
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引用次数: 0

摘要

有证据表明,手术过程会影响中枢神经系统并导致情绪和行为的改变,但人们很少了解急性炎症在促进术后急性焦虑中的作用。本研究旨在探讨右美托咪定(DEX,a2-肾上腺素能受体激动剂)减轻术后急性焦虑的可能机制,这可能与激活海马中的核因子卡巴B(NF-κB)及其下游信号通路有关。实验以大鼠为研究对象,通过高架迷宫和开阔地试验来评估焦虑样行为。阿替帕唑(AT,α2-肾上腺素能受体拮抗剂)抑制DEX,吡咯烷二硫代氨基甲酸(PDTC,抑制IκB磷酸化,阻止NF-κB活化)抑制NF-κB,白细胞介素-6(IL-6)、IL-1β、IL-10和肿瘤坏死因子-α(TNF-α)的水平;海马中 NF-κB 的核转位和焦虑样行为。大鼠在术后6小时和12小时表现出焦虑样行为。术前服用DEX能明显缓解术后焦虑样行为。术前服用DEX可抑制NF-κB的核转位,从而缓解急性术后焦虑。这些研究结果首次表明,大鼠术后急性焦虑可能与海马中的NF-κB核转位有关,而DEX可缓解这种焦虑。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dexmedetomidine alleviates acute postoperative anxiety in rats by suppressing the NF-ĸB pathway.

Evidence suggests that surgical procedures can effect the central nervous system and lead to changes in mood and behavior, rarely understood about the role of acute inflammation in promoting acute anxiety postoperatively. This study was designed to explore the possible mechanism of dexmedetomidine (DEX, a2-adrenergic receptor agonist) for reducing acute postoperative anxiety, which may be related to the activation of nuclear factor kappa B (NF-κB) and downstream signal pathway in the hippocampus. Experiments were conducted with rat, the elevated plus-maze and open field test were performed to evaluate anxiety-like behavior. Inhibit DEX with Atipamezole (AT, α2-adrenergic receptor antagonist) and inhibit NF-κB with Pyrrolidinedithiocarbamate (PDTC, inhibit phosphorylation of IκB, prevent the activation of NF-κB), the level of interleukin-6 (IL-6), IL-1β, IL-10 and Tumor necrosis factor-α (TNF-α); the nuclear translocation of NF-κB in the hippocampus and anxiety-like behavior were measured. Rats exhibited anxiety-like behavior at 6h and 12h after surgery. Preoperative administration of DEX significantly alleviated postoperative anxiety-like behavior. DEX premedication inhibited the nuclear translocation of NF-κB alleviate acute postoperative anxiety. These findings are the first to show that acute postoperative anxiety may be related to NF-κB nuclear translocation in the hippocampus in rats, which can be alleviated by DEX premedication.

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来源期刊
CiteScore
1.40
自引率
12.50%
发文量
211
审稿时长
4.5 months
期刊介绍: Pakistan Journal of Pharmaceutical Sciences (PJPS) is a peer reviewed multi-disciplinary pharmaceutical sciences journal. The PJPS had its origin in 1988 from the Faculty of Pharmacy, University of Karachi as a biannual journal, frequency converted as quarterly in 2005, and now PJPS is being published as bi-monthly from January 2013. PJPS covers Biological, Pharmaceutical and Medicinal Research (Drug Delivery, Pharmacy Management, Molecular Biology, Biochemical, Pharmacology, Pharmacokinetics, Phytochemical, Bio-analytical, Therapeutics, Biotechnology and research on nano particles.
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