脆石斛叶中抗胆固醇化合物的酶法和超声波辅助水溶液两相萃取顺序和同步比较研究

IF 0.7 4区 医学 Q4 PHARMACOLOGY & PHARMACY
Rita Arbianti, Harti Ningsih, Najah Fadilah Putri, Tania Surya Utami, Yuswan Muharam, - Slamet
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引用次数: 0

摘要

应用创新的超声波辅助酶法水溶液两相萃取(UAE-ATPE)方法提高了脆石莲叶的产量,同时探索了顺序萃取和同时萃取两种方法。比较分析包括评估总酚含量(TPC)、总黄酮含量(TFC)、分配系数(k)和回收率(R)。液相色谱-质谱法和扫描电子显微镜对两种技术的提取物进行了评估。与顺序提取相比,同时 UAE-ATPE 的 TPC(5.7±0.1 毫克 GAE/克干叶)和 TFC(3.3±0.1 毫克 QE/克干叶)明显更高,顺序提取的 TPC 和 TFC 分别为 4.5±0.3 毫克 GAE/克干叶和 1.7±0.1 毫克 QE/克干叶。此外,同时进行 UAE-ATPE 萃取可获得更高的酚类和类黄酮化合物 k 值和 R 值。值得注意的是,它鉴定出了与 6 种化合物相对应的 32.4% 的面积,超过了按顺序鉴定出 13 种化合物的 25.3% 的面积。在同时进行的 UAE-ATPE 中观察到了酶水解和超声波萃取的协同效应。在对 HMG-CoA 还原酶的抑制试验中,同步 UAE-ATPE 提取物(200 µg/mL)表现出优异的效果,与顺序提取法的 39.4% 抑制率相比,达到了 66.1% 的优异抑制率。这凸显了 UAE-ATPE 同时提取法在生产浓缩抗胆固醇化合物方面的功效。这项研究有力地证明了同时 UAE-ATPE 法比顺序法的优越性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A comparative study of sequential and simultaneous enzymatic and ultrasound-assisted aqueous two-phase extraction for anticholesterol compounds from Strobilanthes crispus leaves.

An innovative ultrasonic-assisted enzymatic aqueous two-phase extraction (UAE-ATPE) method was applied to enhance the yield from Strobilanthes crispus leaves, exploring both sequential and simultaneous approaches. Comparative analysis included assessing total phenolic content (TPC), total flavonoid content (TFC), partition coefficient (k) and recovery (R). Liquid chromatography-mass spectrometry and scanning electron microscopy evaluated extracts from both techniques. Simultaneous UAE-ATPE demonstrated significantly higher TPC (5.7±0.1 mg GAE/g dry leaves) and TFC (3.3±0.1 mg QE/g dry leaves) compared to sequential extraction, where TPC and TFC measured 4.5±0.3 mg GAE/g dry leaves and 1.7±0.1 mg QE/g dry leaves. Additionally, simultaneous UAE-ATPE yielded higher k and R values for phenolic and flavonoid compounds. Notably, it identified 32.4% of the area corresponding to 6 compounds, surpassing the 25.3% area identified sequentially with 13 compounds. A collaborative effect of enzymatic hydrolysis and ultrasonic extraction was observed in simultaneous UAE-ATPE. In the inhibition test on the HMG-CoA reductase enzyme, simultaneous UAE-ATPE extract (200 µg/mL) exhibited exceptional results, achieving superior inhibition of 66.1% compared to the sequential method's inhibition of 39.4%. This underscores the efficacy of simultaneous UAE-ATPE in producing concentrated anti-cholesterol compounds. The study strongly emphasizes the superiority of simultaneous UAE-ATPE over the sequential approach.

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来源期刊
CiteScore
1.40
自引率
12.50%
发文量
211
审稿时长
4.5 months
期刊介绍: Pakistan Journal of Pharmaceutical Sciences (PJPS) is a peer reviewed multi-disciplinary pharmaceutical sciences journal. The PJPS had its origin in 1988 from the Faculty of Pharmacy, University of Karachi as a biannual journal, frequency converted as quarterly in 2005, and now PJPS is being published as bi-monthly from January 2013. PJPS covers Biological, Pharmaceutical and Medicinal Research (Drug Delivery, Pharmacy Management, Molecular Biology, Biochemical, Pharmacology, Pharmacokinetics, Phytochemical, Bio-analytical, Therapeutics, Biotechnology and research on nano particles.
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