利用 CRISPR-Cas13 进行可编程 RNA 靶向。

IF 3.6 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

RNA Biology Pub Date : 2024-01-01 Epub Date: 2024-05-19 DOI:10.1080/15476286.2024.2351657

Peiguo Shi, Xuebing Wu

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引用次数: 0

摘要

RNA靶向CRISPR-Cas13系统实现了内源性RNA的精确工程，极大地推动了我们对RNA调控的理解以及基于RNA的诊断和治疗应用的开发。本综述旨在总结基于 Cas13 的 RNA 靶向工具和应用，讨论现有工具的局限性和挑战，并提出进一步开发 RNA 靶向系统的潜在方向。

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Programmable RNA targeting with CRISPR-Cas13.

The RNA-targeting CRISPR-Cas13 system has enabled precise engineering of endogenous RNAs, significantly advancing our understanding of RNA regulation and the development of RNA-based diagnostic and therapeutic applications. This review aims to provide a summary of Cas13-based RNA targeting tools and applications, discuss limitations and challenges of existing tools and suggest potential directions for further development of the RNA targeting system.

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来源期刊

RNA Biology 生物-生化与分子生物学

CiteScore

8.60

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： RNA has played a central role in all cellular processes since the beginning of life: decoding the genome, regulating gene expression, mediating molecular interactions, catalyzing chemical reactions. RNA Biology, as a leading journal in the field, provides a platform for presenting and discussing cutting-edge RNA research. RNA Biology brings together a multidisciplinary community of scientists working in the areas of: Transcription and splicing Post-transcriptional regulation of gene expression Non-coding RNAs RNA localization Translation and catalysis by RNA Structural biology Bioinformatics RNA in disease and therapy