用于农药检测的 AChE 固定化磁性生物纳米复合材料(Fe3O4@Cht/Au)的制备和表征。

IF 1.4 4区 农林科学 Q4 ENVIRONMENTAL SCIENCES
Elvan Hasanoğlu Özkan, Nurdan Kurnaz Yetim, Mümin Mehmet Koç
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引用次数: 0

摘要

游离酶在许多应用中都会遇到困难,因为它们不够稳定,在短时间内就会失去活性,最重要的是,虽然它们成本高昂,但在反应中只能使用一次,就会失去作用,而且无法从环境中回收。包覆有生物相容性高分子材料的磁性纳米粒子具有多功能孔隙表面、磁化后易于从环境中清除、在各种恶劣条件下保持稳定等特点,因此有可能成为前景广阔的酶载体。本研究利用掺金的壳聚糖涂层磁性纳米粒子制备了一种基于有机磷农药对乙酰胆碱酯酶抑制作用的候选生物传感器。透射电子显微镜、扫描电子显微镜、X 射线衍射测定法和傅立叶变换红外光谱分析证实了合成的纳米复合材料的结构。使用 VSM 评估了纳米复合材料的磁特性。生物纳米复合材料(Fe3O4@Cht/Au/AChE)用于定性检测环境污染物。对含有机磷废水进行补救是环境可持续发展的一个重要问题。本研究选择敌敌畏和毒死蜱作为有机污染物来评估固定化 Fe3O4@Cht/Au/AChE 的酶活性。确定了 AChE 酶固定化纳米结构(Fe3O4@Cht/Au/AChE)的最佳条件。发现固定化酶的最佳 pH 值为 8,最佳温度为 60 °C。在第 20 次重复使用时,发现固定化酶的保留活性约为 50%。在含有 150 µL 杀虫剂的情况下,固定化酶活性的保留率约为 25%。对杀虫剂进行了方法验证。使用固定化乙酰胆碱酯酶时,敌敌畏和毒死蜱的 LOD(检测限)-LOQ(定量限)值范围分别为 0.0087-0.029 nM 和 0.0014-0.0046 nM。作为精密度指标的相对标准偏差(RSD%)值低于 2%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Preparation and characterization of AChE immobilized magnetic bio-nanocomposites (Fe3O4@Cht/Au) for pesticide detection.

Free enzymes cause difficulties in many applications due to their insufficient stability, loss of activity in a short time, and most importantly, although they are costly, they are used only once in reactions, lose their effect and cannot be recovered from the environment. Magnetic nanoparticles coated with biocompatible polymeric material are potential candidates for promising enzyme carriers due to their multifunctional pore surfaces, easy removal from the environment provided by the magnetization, ability to main stability under various harsh conditions. This study prepared a biosensor candidate based on the inhibiting acetylcholinesterase enzyme by organophosphate pesticides from chitosan-coated magnetic nanoparticles doped with gold. Transmission electron microscopy, scanning electron microscopy, X-ray diffraction diffractometry, and Fourier transform infrared spectroscopy analysis confirmed the structure of synthesized nanocomposites. Magnetic characteristics of the nanocomposites were assessed using VSM. Bio-nanocomposite (Fe3O4@Cht/Au/AChE) was used to determine environmental pollutants qualitatively. Remediation of organophosphate-containing wastewater is an essential issue for environmental sustainability. In this work, Dichlorvos and Chlorpyrifos were selected as organic pollutants to assess the enzymatic activity of immobilized Fe3O4@Cht/Au/AChE. Optimum conditions for AChE enzyme were immobilized nanostructures (Fe3O4@Cht/Au/AChE) were determined. The optimum pH for the immobilized enzyme was found to be 8, and the optimum temperature was found to be 60 °C. Retained immobilized enzyme activity is found to be around 50% for the 20th reuse. In the presence of 150 µL pesticide, retained immobilized enzyme activity is found to be around 25%. Method validation was performed for pesticides. When using immobilized AChE, the LOD (limit of detection)-LOQ (limit of quantitation) values for Dichlorovos and Chlorpyrifos was obtained in the range of 0.0087-0.029 nM and 0.0014-0.0046 nM, respectively. The relative standard deviation (RSD%) values, which are indicators of precision, were found to be below 2%.

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来源期刊
CiteScore
4.00
自引率
5.00%
发文量
87
审稿时长
1 months
期刊介绍: 12 issues per year Abstracted/indexed in: Agricola; Analytical Abstracts; ASFA 3: Aquatic Pollution & Environmental Quality; BioSciences Information Service of Biological Abstracts (BIOSIS); CAB Abstracts; CAB AGBiotech News and Information; CAB Irrigation & Drainage Abstracts; CAB Soils & Fertilizers Abstracts; Chemical Abstracts Service Plus; CSA Aluminum Industry Abstracts; CSA ANTE: Abstracts in New Technology and Engineering; CSA ASFA 3 Aquatic Pollution and Environmental Quality; CSA ASSIA: Applied Social Sciences Index & Abstracts; CSA Ecology Abstracts; CSA Entomology Abstracts; CSA Environmental Engineering Abstracts; CSA Health & Safety Science Abstracts; CSA Pollution Abstracts; CSA Toxicology Abstracts; CSA Water Resource Abstracts; EBSCOhost Online Research Databases; Elsevier BIOBASE/Current Awareness in Biological Sciences; Elsevier Engineering Information: EMBASE/Excerpta Medica/ Engineering Index/COMPENDEX PLUS; Environment Abstracts; Environmental Knowledge; Food Science and Technology Abstracts; Geo Abstracts; Geobase; Food Science; Index Medicus/ MEDLINE; INIST-Pascal/ CNRS; NIOSHTIC; ISI BIOSIS Previews; Pesticides; Food Contaminants and Agricultural Wastes: Analytical Abstracts; Pollution Abstracts; PubSCIENCE; Reference Update; Research Alert; Science Citation Index Expanded (SCIE); and Water Resources Abstracts.
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