胆固醇环糊精可提高冷冻斑马鱼（Danio rerio）精子的运动能力和存活率

IF 2.3 3区生物学 Q2 BIOLOGY

Cryobiology Pub Date : 2024-05-25 DOI:10.1016/j.cryobiol.2024.104909

Jennifer L. Matthews, Joy M. Murphy, Zoltan M. Varga

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引用次数: 0

摘要

我们利用胆固醇负载甲基-β-环糊精（CLC）和非负载甲基-β-环糊精（MβC）研究了调节斑马鱼精子质膜中胆固醇水平的影响。在冷冻保存斑马鱼精子之前用这些物质对其进行处理，并对处理过和未处理的样本进行解冻后精子活力和体外受精（IVF）率的比较。我们的研究结果表明，在每 1.2 x 108 个细胞 0.5 至 4.0 毫克的浓度范围内，向精子膜中添加胆固醇可提高解冻后的运动能力、运动细胞数量和运动细胞存活率。相反，使用 MβC 以每 1.2 x 108 个细胞 1.0 和 2.0 毫克的浓度消耗胆固醇则会降低这些参数。与未经处理的精子相比，所有经过 CLC 处理的精子样本的试管受精率平均提高了 15%。在冷冻保存前将 CLC 加入扩展剂中有利于斑马鱼解冻后精子的数量和质量。

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Cholesterol-loaded cyclodextrin improves motility and survival of cryopreserved zebrafish (Danio rerio) sperm

We studied the impact of modulating cholesterol levels in zebrafish sperm plasma membranes using cholesterol-loaded methyl-β-cyclodextrin (CLC) and unloaded methyl-β-cyclodextrin (MβC). Zebrafish sperm were treated with these substances before cryopreservation, and post-thaw sperm motility and in vitro fertilization (IVF) rates were compared between treated and untreated samples. Our findings indicate that adding cholesterol to sperm membranes increases post-thaw motility, motile cell count, and motile cell survival within a 0.5–4.0 mg per 1.2 × 10⁸ cell concentration range. Conversely, depleting cholesterol using MβC at 1.0 and 2.0 mg per 1.2 × 10⁸ cells reduced these parameters. On average, all CLC-treated sperm samples produced a 15 % higher IVF rate compared to untreated sperm. Including CLC in the extender before cryopreservation is beneficial for post-thaw sperm quantity and quality in zebrafish.

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来源期刊

Cryobiology 生物-生理学

CiteScore

5.40

自引率

7.40%

发文量

审稿时长

56 days

期刊介绍： Cryobiology: International Journal of Low Temperature Biology and Medicine publishes research articles on all aspects of low temperature biology and medicine. Research Areas include: • Cryoprotective additives and their pharmacological actions • Cryosurgery • Freeze-drying • Freezing • Frost hardiness in plants • Hibernation • Hypothermia • Medical applications of reduced temperature • Perfusion of organs • All pertinent methodologies Cryobiology is the official journal of the Society for Cryobiology.