17 型免疫反应促进牙周炎中口腔上皮细胞的增殖

IF 2.2 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE
Ying Zhou , Die Lv , Weideng Wei , Tong Zhou , Shijie Tang , Fan Yang , Jiuge Zhang , Lanxin Jiang , Xiaoqiang Xia , Yuchen Jiang , Qianming Chen , Yuan Yue , Xiaodong Feng
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引用次数: 0

摘要

设计利用时间依赖性结扎诱导的牙周炎小鼠模型来探讨牙龈增生和白细胞介素17A(IL-17A)阳性细胞的浸润。免疫组化和流式细胞术用于确定 IL-17A 在结扎诱导的牙周炎模型中的定位和表达。为了评估 IL-17A 的表达水平,我们重新分析了先前存在的单细胞 RNA 测序数据集,并将牙周炎患者与健康患者进行了比较。我们检测了牙周炎模型中牙龈和舌头上皮细胞的增殖标记物,包括增殖细胞核抗原(PCNA)、转录信号转导和激活因子(STAT3)、Yes 相关蛋白(YAP)和 c-JUN。每天注射抗IL-17A制剂,观察牙周炎模型中口腔黏膜的增殖变化。结果结扎诱导的牙周炎模型表现出明显的 IL-17A 阳性细胞浸润,同时牙龈和舌头上皮的厚度显著增加。IL-17A 会引发人类正常口腔角质细胞的增殖,并伴随 PCNA、STAT3、YAP 和 c-JUN 的上调。结论:这些研究结果表明,17 型免疫反应在应对牙周炎时会促进口腔上皮细胞的增殖,从而突显了其在维护口腔上皮屏障中的关键作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Type 17 immune response promotes oral epithelial cell proliferation in periodontitis

Objectives

This study aims to investigate the effects of type 17 immune response on the proliferation of oral epithelial cells in periodontitis.

Design

A time-dependent ligature induced periodontitis mouse model was utilized to explore gingival hyperplasia and the infiltration of interleukin 17A (IL-17A) positive cells. Immunohistochemistry and flow cytometry were employed to determine the localization and expression of IL-17A in the ligature induced periodontitis model. A pre-existing single-cell RNA sequencing dataset, comparing individuals affected by periodontitis with healthy counterparts, was reanalyzed to evaluate IL-17A expression levels. We examined proliferation markers, including proliferating cell nuclear antigen (PCNA), signal transducer and activator of transcription (STAT3), Yes-associated protein (YAP), and c-JUN, in the gingival and tongue epithelium of the periodontitis model. An anti-IL-17A agent was administered daily to observe proliferative changes in the oral mucosa within the periodontitis model. Cell number quantification, immunofluorescence, and western blot analyses were performed to assess the proliferative responses of human normal oral keratinocytes to IL-17A treatment in vitro.

Results

The ligature induced periodontitis model exhibited a marked infiltration of IL-17A-positive cells, alongside significant increase in thickness of the gingival and tongue epithelium. IL-17A triggers the proliferation of human normal oral keratinocytes, accompanied by upregulation of PCNA, STAT3, YAP, and c-JUN. The administration of an anti-IL-17A agent attenuated the proliferation in oral mucosa.

Conclusions

These findings indicate that type 17 immune response, in response to periodontitis, facilitates the proliferation of oral epithelial cells, thus highlighting its crucial role in maintaining the oral epithelial barrier.

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来源期刊
Archives of oral biology
Archives of oral biology 医学-牙科与口腔外科
CiteScore
5.10
自引率
3.30%
发文量
177
审稿时长
26 days
期刊介绍: Archives of Oral Biology is an international journal which aims to publish papers of the highest scientific quality in the oral and craniofacial sciences. The journal is particularly interested in research which advances knowledge in the mechanisms of craniofacial development and disease, including: Cell and molecular biology Molecular genetics Immunology Pathogenesis Cellular microbiology Embryology Syndromology Forensic dentistry
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