纵向结构磁共振成像检测到多重压力诱导成年雄性大鼠杏仁核海马体积缩小

IF 4 Q2 NEUROSCIENCES

Biological psychiatry global open science Pub Date : 2024-05-15 DOI:10.1016/j.bpsgos.2024.100334

Rie Ryoke, Teruo Hashimoto, Ryuta Kawashima

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引用次数: 0

摘要

背景创伤事件会导致长期无法控制的恐惧和焦虑。创伤后应激障碍是一种难治性精神障碍，利用动物模型研究神经生物学机制有望有助于创伤后应激障碍治疗的发展。在这项研究中，我们将多重应激（MS）和纵向体内磁共振成像相结合，揭示了长期焦虑样行为对成年雄性大鼠大脑的影响。方法将12只雄性Wistar大鼠（8周大）置于1毫安脚震和强迫游泳的MS中，同时将12只对照组大鼠置于塑料笼中。两组大鼠均在接触 MS 15 天后，在不同于 MS 的情境中进行 0.1 毫安脚震的情境恐惧条件反射。分别在 24 小时、9 天和 16 天后进行了三次保持测试。进行了两次磁共振成像扫描，一次是在诱导 MS 的前一天，另一次是在第三次保持测试的第二天，间隔时间为 32 天。基于体素的全脑形态计量分析显示，与对照组大鼠相比，MS 组大鼠杏仁核海马前区灰质体积减少。这些结果表明，杏仁海马区的个体差异可能与严重应激后的持久恐惧反应有关。

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Multiple Stressors Induce Amygdalohippocampal Volume Reduction in Adult Male Rats as Detected by Longitudinal Structural Magnetic Resonance Imaging

Background

Traumatic events can cause long-lasting and uncontrollable fear and anxiety. Posttraumatic stress disorder is an intractable mental disorder, and neurobiological mechanisms using animal models are expected to help development of posttraumatic stress disorder treatment. In this study, we combined multiple stress (MS) and longitudinal in vivo magnetic resonance imaging to reveal the effects of long-lasting anxiety-like behaviors on adult male rat brains.

Methods

Twelve male Wistar rats (8 weeks old) were exposed to the MS of 1-mA footshocks and forced swimming, while 12 control rats were placed in a plastic cage. Contextual fear conditioning with 0.1-mA footshocks in a context different from the MS was conducted 15 days after the MS for both groups. Three retention tests were administered after 24 hours and 9 and 16 days. Two magnetic resonance imaging scans were conducted, one on the day before MS induction and one the day after the third retention test, with a 32-day interval.

Results

The MS group showed greater freezing responses than the control group in all retention tests. Whole-brain voxel-based morphometry analyses revealed reduced gray matter volume in the anterior amygdalohippocampal area in MS group rats compared with control rats. These volume changes were negatively associated with freezing time in the third retention test in the MS group.